Tbx16 regulates hox gene activation in mesodermal progenitor cells
- Payumo, A.Y., McQuade, L.E., Walker, W.J., Yamazoe, S., Chen, J.K.
- Nature Chemical Biology 12(9): 694-701 (Journal)
- Registered Authors
- Chen, James K.
- Chemical tools, Developmental biology, Networks and systems biology, Transcription
- MeSH Terms
- Genes, Homeobox/genetics*
- Molecular Structure
- Stem Cells/cytology
- Stem Cells/metabolism*
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- 27376691 Full text @ Nat. Chem. Biol.
Payumo, A.Y., McQuade, L.E., Walker, W.J., Yamazoe, S., Chen, J.K. (2016) Tbx16 regulates hox gene activation in mesodermal progenitor cells. Nature Chemical Biology. 12(9):694-701.
The transcription factor T-box 16 (Tbx16, or Spadetail) is an essential regulator of paraxial mesoderm development in zebrafish (Danio rerio). Mesodermal progenitor cells (MPCs) fail to differentiate into trunk somites in tbx16 mutants and instead accumulate within the tailbud in an immature state. However, the mechanisms by which Tbx16 controls mesoderm patterning have remained enigmatic. We describe here the use of photoactivatable morpholino oligonucleotides to determine the Tbx16 transcriptome in MPCs. We identified 124 Tbx16-regulated genes that were expressed in zebrafish gastrulae, including several developmental signaling proteins and regulators of gastrulation, myogenesis and somitogenesis. Unexpectedly, we observed that a loss of Tbx16 function precociously activated posterior hox genes in MPCs, and overexpression of a single posterior hox gene was sufficient to disrupt MPC migration. Our studies support a model in which Tbx16 regulates the timing of collinear hox gene activation to coordinate the anterior-posterior fates and positions of paraxial MPCs.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes