PUBLICATION

A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish

Authors
Hu, W., Zeng, L., Wang, Y., Liu, Z., Ye, X., Li, C.
ID
ZDB-PUB-160630-6
Date
2016
Source
The Analyst   141(18): 5450-5 (Journal)
Registered Authors
Hu, Wei
Keywords
none
MeSH Terms
  • Animals
  • Fluorescent Dyes*
  • Fluorides/analysis*
  • HeLa Cells
  • Humans
  • Microscopy*
  • Photons
  • Zebrafish
PubMed
27353376 Full text @ Analyst
Abstract
Using 6-hydroxyl-quinoline-2-benzothiazole () as a two-photon fluorophore and tert-butyldiphenylsilyl as a recognition domain for F(-), a ratiometric two-photon fluorescent fluoride probe, , was synthesized and fully characterized. displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution. was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved. has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping