PUBLICATION
A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish
- Authors
- Hu, W., Zeng, L., Wang, Y., Liu, Z., Ye, X., Li, C.
- ID
- ZDB-PUB-160630-6
- Date
- 2016
- Source
- The Analyst 141(18): 5450-5 (Journal)
- Registered Authors
- Hu, Wei
- Keywords
- none
- MeSH Terms
-
- Humans
- Photons
- Fluorescent Dyes*
- Zebrafish
- HeLa Cells
- Microscopy*
- Animals
- Fluorides/analysis*
- PubMed
- 27353376 Full text @ Analyst
Citation
Hu, W., Zeng, L., Wang, Y., Liu, Z., Ye, X., Li, C. (2016) A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish. The Analyst. 141(18):5450-5.
Abstract
Using 6-hydroxyl-quinoline-2-benzothiazole () as a two-photon fluorophore and tert-butyldiphenylsilyl as a recognition domain for F(-), a ratiometric two-photon fluorescent fluoride probe, , was synthesized and fully characterized. displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution. was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved. has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping