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ZFIN ID: ZDB-PUB-160625-8
Impaired constitutive and regenerative neurogenesis in adult hyperglycemic zebrafish
Dorsemans, A.C., Soulé, S., Weger, M., Bourdon, E., Lefebvre d'Hellencourt, C., Meilhac, O., Diotel, N.
Date: 2017
Source: The Journal of comparative neurology   525(3): 442-458 (Journal)
Registered Authors: Diotel, Nicolas, Weger, Meltem
Keywords: Key-words: blood-brain barrier, RRID: AB_10049650, RRID: AB_141372, RRID: AB_141607, RRID: AB_2160651, RRID: AB_2314535, RRID: SCR_014329, brain repair, diabetes, hyperglycemia, neural stem cells, neurogenesis, pro-inflammatory cytokines, radial glial cells, stab wound injury, teleost, zebrafish
MeSH Terms:
  • Acute Disease
  • Animals
  • Brain/pathology*
  • Brain/physiopathology*
  • Brain Injuries, Traumatic/pathology
  • Brain Injuries, Traumatic/physiopathology
  • Chronic Disease
  • Disease Models, Animal
  • Encephalitis/pathology
  • Encephalitis/physiopathology
  • Female
  • Gene Expression Regulation/physiology
  • Glucose
  • Head Injuries, Penetrating/pathology
  • Head Injuries, Penetrating/physiopathology
  • Hyperglycemia/pathology*
  • Hyperglycemia/physiopathology*
  • Male
  • Nerve Regeneration/physiology*
  • Neurogenesis/physiology*
  • Wound Healing/physiology
  • Wounds, Stab/pathology
  • Wounds, Stab/physiopathology
  • Zebrafish
PubMed: 27339277 Full text @ J. Comp. Neurol.
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ABSTRACT
A growing body of evidence supports hyperglycemia as a putative contributor to several brain dysfunctions observed in diabetes patients, such as impaired memory capacity, neural plasticity, and neurogenic processes. Thanks to the persistence of radial glial cells acting as neural stem cells, the brain of the adult zebrafish constitutes a relevant model to investigate constitutive and injury-induced neurogenesis in adult vertebrates. However, there is limited understanding of the impact of hyperglycemia on brain dysfunction in the zebrafish model. This work aimed at exploring the impact of acute and chronic hyperglycemia on brain homeostasis and neurogenesis. Acute hyperglycemia was shown to promote gene expression of pro-inflammatory cytokines (il1β, il6, il8, and tnfα) in the brain and chronic hyperglycemia to impair expression of genes involved in the establishment of the blood-brain barrier (claudin 5a, zona occludens 1a and b). Chronic hyperglycemia also decreased brain cell proliferation in most neurogenic niches throughout the forebrain and the midbrain. By using a stab wound telencephalic injury model, the impact of hyperglycemia on brain repair mechanisms was investigated. Whereas the initial step of parenchymal cell proliferation was not affected by acute hyperglycemia, later proliferation of neural progenitors was significantly decreased by chronic hyperglycemia in the injured brain of fish. Taken together, these data offer new evidence highlighting the evolutionary-conserved adverse effects of hyperglycemia on neurogenesis and brain healing in zebrafish. In addition, our study reinforces the utility of zebrafish as a robust model for studying the effects of metabolic disorders on the central nervous system. This article is protected by copyright. All rights reserved.
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