PUBLICATION
Beneficial roles of melanoma cell adhesion molecule in spinal cord transection recovery in adult zebrafish
- Authors
- Liu, C.J., Xie, L., Cui, C., Chu, M., De Zhao, H., Yao, L., Li, Y.H., Schachner, M., Shen, Y.Q.
- ID
- ZDB-PUB-160619-2
- Date
- 2016
- Source
- Journal of neurochemistry 139(2): 187-196 (Journal)
- Registered Authors
- Schachner, Melitta
- Keywords
- MCAM, blood vessels, inflammation, regeneration, spinal cord injury, zebrafish
- MeSH Terms
-
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- Morpholines/pharmacology
- RNA, Messenger/biosynthesis
- RNA, Messenger/genetics
- Inflammation Mediators/physiology
- Axons/drug effects
- Axons/pathology
- Locomotion
- Spinal Cord Injuries/genetics*
- Spinal Cord Injuries/pathology*
- Animals, Genetically Modified
- Swimming
- CD146 Antigen/genetics*
- CD146 Antigen/metabolism
- Cell Count
- Animals
- Motor Neurons/drug effects
- Zebrafish
- Angiogenesis Modulating Agents/pharmacology
- Blood Vessels/metabolism
- Recovery of Function
- PubMed
- 27318029 Full text @ J. Neurochem.
Citation
Liu, C.J., Xie, L., Cui, C., Chu, M., De Zhao, H., Yao, L., Li, Y.H., Schachner, M., Shen, Y.Q. (2016) Beneficial roles of melanoma cell adhesion molecule in spinal cord transection recovery in adult zebrafish. Journal of neurochemistry. 139(2):187-196.
Abstract
Melanoma cell adhesion molecule (MCAM) is a multifunctional protein involved in miscellaneous processes, including development and tumor angiogenesis. Here, spinal cord transection in adult zebrafish was used to investigate the effects of MCAM on spinal cord injury (SCI) and subsequent recovery. Expression of MCAM mRNA increased and co-localized with motoneurons in the spinal cord after SCI. With MCAM morpholino (MO) treatment, inhibition of MCAM retarded both axon regrowth and locomotor recovery in the spinal cord injured zebrafish. Further, MCAM mRNA expression was also observed in fli1a:EGFP transgenic zebrafish, which specifically show labeled blood vessels. Inhibition of MCAM down-regulated the expression of angiogenesis-related factors, such as VEGFR2, p-p38 and p-AKT, and the inflammatory factors TNF-α, IL-1β and IL-8. Taken together, these data suggest that MCAM may have a beneficial role in the recovery from SCI, via the promotion of neurogenesis and angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping