ZFIN ID: ZDB-PUB-160618-2
Roundup in Zebrafish: Effects on Oxidative Status and Gene Expression
Velasques, R.R., Zomer Sandrini, J., da Rosa, C.E.
Date: 2016
Source: Zebrafish   13(5): 432-41 (Journal)
Registered Authors: da Rosa, Carlos Eduardo
Keywords: none
MeSH Terms:
  • Animals
  • Female
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Gene Expression/drug effects*
  • Gills/drug effects
  • Gills/metabolism
  • Glycine/analogs & derivatives*
  • Glycine/toxicity
  • Herbicides/toxicity
  • Liver/drug effects
  • Liver/metabolism
  • Male
  • Oxidative Stress*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 27315435 Full text @ Zebrafish
The objective of the present study was to determine the effects of Roundup(®) on oxidative status in adult Danio rerio liver and gills. Reactive oxygen species (ROS) and antioxidant capacity (ACAP) were measured in fish after exposure to Roundup (5 and 10 mg/L) for 24, 48, 72, and 96 h. Furthermore, gene expression related to antioxidant response was evaluated after 24 and 96 h. In gills, an increase in ACAP was observed after 96 h in the highest concentration. In the liver, a reduction in ROS and ACAP was observed after 24 h, whereas an increase in ACAP was observed after 48 h in the highest concentration. Exposure to the lowest concentration caused a reduction in ROS after 72 and 96 h. Regarding gene expression, a reduction in superoxide dismutase 2 (sod2) and glutathione S-transferase (gstπ) was observed. An increase in uncoupling protein 1 (ucp1) expression was observed in gills of animals exposed to the highest concentration after 24 h. Glutathione peroxidase (gpx) gene expression was reduced in the gills of animals exposed to the lowest concentration; however, it was induced in liver tissue after 96 h of exposure to the highest concentration. These results indicate that zebrafish exposure to Roundup alters oxidative status and causes a response in terms of antioxidant defense system gene expression.