PUBLICATION
Chemical approaches to angiogenesis in development and regeneration
- Authors
- Zhao, H., Huang, H., Lin, S.
- ID
- ZDB-PUB-160618-13
- Date
- 2016
- Source
- Methods in cell biology 134: 369-76 (Chapter)
- Registered Authors
- Huang, Haigen, Lin, Shuo
- Keywords
- Intersegmental blood vessels, Primary cells, Proangiogenesis, Small molecules, Transgenic zebrafish, VEGF receptor inhibitor
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Embryonic Development/genetics*
- Endothelial Cells/cytology
- Morphogenesis/genetics*
- Neovascularization, Physiologic/genetics*
- Regeneration/genetics*
- Signal Transduction
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 27312498 Full text @ Meth. Cell. Biol.
Citation
Zhao, H., Huang, H., Lin, S. (2016) Chemical approaches to angiogenesis in development and regeneration. Methods in cell biology. 134:369-76.
Abstract
Vascular endothelial cells are essential building blocks of angiogenesis, which is required for normal embryonic development and tissue regeneration. In this chapter, we describe how to use transgenic zebrafish embryos expressing vascular-specific green fluorescent protein to evaluate differentiation, growth, and morphogenesis of endothelial cells. When combined with instrument automation and computational analysis, this method allows high-throughput screening for biologically active small chemical molecules that are effective in promoting angiogenesis. These molecules can be validated in mammalian endothelial cell differentiation and proliferation assays. These studies provide new reagents and therapeutic candidates for regenerative medicine studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping