PUBLICATION

Real-time 3D visualization of cellular rearrangements during cardiac valve formation

Authors
Pestel, J., Ramadass, R., Gauvrit, S., Helker, C., Herzog, W., Stainier, D.Y.
ID
ZDB-PUB-160616-6
Date
2016
Source
Development (Cambridge, England)   143: 2217-27 (Journal)
Registered Authors
Helker, Christian, Herzog, Wiebke, Stainier, Didier
Keywords
Apc, Cardiac valves, Endocardium, Live imaging, Notch, Wnt, Zebrafish
MeSH Terms
  • Animals
  • Cell Movement
  • Coronary Circulation
  • Endocardium/cytology
  • Endocardium/embryology
  • Gene Expression Regulation, Developmental
  • Heart Atria/cytology
  • Heart Atria/embryology
  • Heart Valves/cytology*
  • Heart Valves/embryology*
  • Heart Ventricles/cytology
  • Heart Ventricles/embryology
  • Imaging, Three-Dimensional*
  • Mutation/genetics
  • Myocardial Contraction
  • Organogenesis/genetics
  • Receptors, Notch/metabolism
  • Wnt Signaling Pathway
  • Zebrafish
PubMed
27302398 Full text @ Development
Abstract
During cardiac valve development, the single-layered endocardial sheet at the atrioventricular canal (AVC) is remodeled into multilayered immature valve leaflets. Most of our knowledge about this process comes from examining fixed samples that do not allow a real-time appreciation of the intricacies of valve formation. Here, we exploit non-invasive in vivo imaging techniques to identify the dynamic cell behaviors that lead to the formation of the immature valve leaflets. We find that in zebrafish, the valve leaflets consist of two sets of endocardial cells at the luminal and abluminal side, which we refer to as luminal cells (LCs) and abluminal cells (ALCs), respectively. By analyzing cellular rearrangements during valve formation, we observed that the LCs and ALCs originate from the atrium and ventricle, respectively. Furthermore, we utilized Wnt/β-catenin and Notch signaling reporter lines to distinguish between the LCs and ALCs, and also found that cardiac contractility and/or blood flow is necessary for the endocardial expression of these signaling reporters. Thus, our 3D analyses of cardiac valve formation in zebrafish provide fundamental insights into the cellular rearrangements underlying this process.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes