PUBLICATION
Solute Carriers (SLCs) identified and characterized from kidney transcriptome of golden mahseer (Tor putitora) (Fam: Cyprinidae)
- Authors
- Barat, A., Sahoo, P.K., Kumar, R., Pande, V.
- ID
- ZDB-PUB-160612-10
- Date
- 2016
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 200: 54-61 (Journal)
- Registered Authors
- Keywords
- glucose transporters, golden mahseer, molecular characterization, solute carriers
- MeSH Terms
-
- Ammonium Compounds/metabolism
- Animals
- Bile Acids and Salts/metabolism
- Biogenic Amines/metabolism
- Biological Transport
- Choline/metabolism
- Cyprinidae/genetics*
- Cyprinidae/metabolism*
- Gene Expression Profiling*
- Gene-Environment Interaction
- Genomics
- Glucose/metabolism
- Humans
- Kidney/metabolism*
- Membrane Transport Proteins/genetics*
- Membrane Transport Proteins/metabolism
- Mitochondrial Membranes/metabolism
- Neurotransmitter Agents/metabolism
- Organ Specificity
- Phylogeny
- Urea/metabolism
- Vitamins/metabolism
- PubMed
- 27287540 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Barat, A., Sahoo, P.K., Kumar, R., Pande, V. (2016) Solute Carriers (SLCs) identified and characterized from kidney transcriptome of golden mahseer (Tor putitora) (Fam: Cyprinidae). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 200:54-61.
Abstract
The solute carriers (SLC) are trans-membrane proteins, those regulate the transport of various substances (sugars, amino acids, nucleotides, inorganic cations/anions, metals, drugs etc.) across the cell membrane. There are more than 338 solute carriers (SLC) reported in fishes that play crucial role in cellular influx and efflux. The study of solute carrier families may reveal many answers regarding the function of transporter genes in the species and their effect in the existing environment. Therefore, we performed RNA sequencing of kidney tissue of the golden mahseer (Tor putitora) using Illumina platform to identify the solute carrier families and characterized 24 putative functional genes under 15 solute carrier families. Out of 24 putative functional genes, 11 genes were differentially expressed in different tissues (head kidney, trunk kidney, spleen, liver, gill, muscle, intestine and brain) using qRT-PCR assay. The slc5a1, slc5a12, slc12a3, slc13a3, slc22a13 and slc26a6 were highly expressed in kidney. The slc15a2, slc25a47, slc33a1 and slc38a2 were highly expressed in brain and slc30a5 was over-expressed in gill. The unrooted phylogenetic trees of slc2, slc5, slc13 and slc33 were constructed using amino acid sequences of Homo sapiens, Salmo salar, Danio rerio, Cyprinus carpio and Tor putitora. It appears that all the putative solute carrier families are very much conserved in human and fish species including the present fish, golden mahseer. This study provides the first hand database of solute carrier families particularly transporter encoding proteins in the species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping