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ZFIN ID: ZDB-PUB-160611-8
Characterization of GPR101 transcripts structure and expression patterns
Trivellin, G., Bjelobaba, I., Daly, A.F., Larco, D.O., Palmeira, L., Faucz, F.R., Thiry, A., Leal, L.F., Rostomyan, L., Quezado, M., Schernthaner-Reiter, M.H., Janjic, M., Villa, C., Wu, T.J., Stojilkovic, S.S., Albert, B., Feldman, B., Stratakis, C.A.
Date: 2016
Source: Journal of molecular endocrinology 57(2): 97-111 (Journal)
Registered Authors: Feldman, Benjamin
Keywords: none
MeSH Terms:
  • Adult
  • Animals
  • Computational Biology/methods
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Gene Expression Regulation, Developmental
  • Humans
  • Macaca mulatta
  • Male
  • Organ Specificity/genetics
  • Pituitary Gland/metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger/chemistry
  • RNA, Messenger/genetics*
  • Rats
  • Receptors, G-Protein-Coupled/genetics*
  • Untranslated Regions
  • Zebrafish
PubMed: 27282544 Full text @ J. Mol. Endocrinol.
We recently showed that Xq26.3 microduplications cause X-linked acrogigantism (X-LAG). X-LAG patients mainly present with growth hormone and prolactin-secreting adenomas and share a minimal duplicated region containing at least four genes. GPR101 was the only gene highly expressed in their pituitary lesions, but little is known about its expression patterns. GPR101 transcripts were characterized in human tissues by 5'-RACE and RNAseq, while the putative promoter was bioinformatically predicted. We investigated GPR101 mRNA and protein expression by RT-qPCR, whole-mount in situ hybridization, and immunostaining, in human, rhesus monkey, rat, and zebrafish. We identified four GPR101 isoforms characterized by different 5' untranslated regions (UTRs) and a common 6.1 kb-long 3'UTR. GPR101 expression was very low or absent in almost all adult human tissues examined, except for specific brain regions. Strong GPR101 staining was observed in human fetal pituitary and during adolescence, whereas very weak/absent expression was detected during childhood and adult life. In contrast to humans, adult pituitaries of monkey and rat expressed GPR101, but in different cell types. Gpr101 is expressed in the brain and pituitary during rat and zebrafish development; in rat pituitary Gpr101 is expressed only after birth and showed sexual dimorphism. This study shows that different GPR101 transcripts exist and that the brain is the major site of GPR101 expression across different species, although divergent species- and temporal-specific expression patterns are evident. These findings suggest an important role for GPR101 in brain and pituitary development and likely reflect the very different growth, development and maturation patterns among species.