PUBLICATION
Detection of exposure effects of mixtures of heavy polycyclic aromatic hydrocarbons in zebrafish embryos
- Authors
- Barranco, A., Escudero, L., Sanz Landaluze, J., Rainieri, S.
- ID
- ZDB-PUB-160611-6
- Date
- 2017
- Source
- Journal of applied toxicology : JAT 37(3): 253-264 (Journal)
- Registered Authors
- Keywords
- PAHs, bioaccumulation, embryos, gene expression, mixtures, toxicity, zebrafish
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects*
- Embryonic Development/genetics
- Gene Expression/drug effects
- Polycyclic Aromatic Hydrocarbons/chemistry
- Polycyclic Aromatic Hydrocarbons/metabolism
- Polycyclic Aromatic Hydrocarbons/toxicity*
- Water Pollutants, Chemical/chemistry
- Water Pollutants, Chemical/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- PubMed
- 27283969 Full text @ J. Appl. Toxicol.
- CTD
- 27283969
Citation
Barranco, A., Escudero, L., Sanz Landaluze, J., Rainieri, S. (2017) Detection of exposure effects of mixtures of heavy polycyclic aromatic hydrocarbons in zebrafish embryos. Journal of applied toxicology : JAT. 37(3):253-264.
Abstract
In this study we evaluated the exposure effects of mixtures of five polycyclic aromatic hydrocarbons (PAHs); namely, benzo[a]anthracene, benzo[a]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene and chrysene on zebrafish embryos. Supplementation of the exposure media with 0.45% dimethyl sulfoxide and 50 ppm of Tween 20 could guarantee the solubilization and stabilization of the PAHs up to 24 h without affecting the embryos development. The exposure effects were tested by detecting the differential expression of a number of genes related to the aryl hydrocarbon receptor gene battery. Effects were detectable already after 6 h of exposure. After 24 h of exposure, all PAHs, except for benzo[a]anthracene, acted as potent inducers of the gene cyp1a1. Benzo[k]fluoranthene was the major inducer; the effect caused by the mixture at the lower concentration tested (1 ng ml(-1) ) was dominated by its presence. However, in the mixture at the highest concentration tested (10 ng ml(-1) ) it caused less induction and was not dominant. No significant bioaccumulation values were detected on embryos exposed to the PAHs tested in this study; however, the results obtained, indicated that PAHs undergo a very rapid metabolization inside the embryos, and that those biotransformation products yield changes on the expression of genes involved in the aryl hydrocarbon receptor pathway. Future work should focus on identification of the PAH metabolization products and on the effect of these metabolites on toxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping