PUBLICATION

miR-219 regulates neural progenitors by dampening apical Par protein-dependent Hedgehog signaling

Authors
Hudish, L.I., Galati, D.F., Ravanelli, A.M., Pearson, C.G., Huang, P., Appel, B.
ID
ZDB-PUB-160527-4
Date
2016
Source
Development (Cambridge, England)   143(13): 2292-304 (Journal)
Registered Authors
Appel, Bruce, Huang, Peng, Ravanelli, Andrew M.
Keywords
MicroRNA, Polarity, Hedgehog, Neural progenitors, Zebrafish
MeSH Terms
  • Animals
  • Cell Count
  • Cell Polarity
  • Cilia/metabolism
  • Embryo, Nonmammalian/metabolism
  • Hedgehog Proteins/metabolism*
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Mutation/genetics
  • Neural Stem Cells/cytology*
  • Neural Stem Cells/metabolism*
  • Organogenesis
  • Signal Transduction*
  • Zebrafish/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27226318 Full text @ Development
Abstract
The transition of dividing neuroepithelial progenitors to differentiated neurons and glia is essential to the formation of a functional nervous system. Sonic Hedgehog (Shh) is a mitogen for spinal cord progenitors, but how cells become insensitive to the proliferative effects of Shh is not well understood. Because Shh reception occurs at primary cilia, which are positioned within the apical membrane of neuroepithelial progenitors, we hypothesized that loss of apical characteristics reduces Shh signaling response, causing cell cycle exit and differentiation. We tested this hypothesis using genetic and pharmacological manipulation, gene expression analysis and time-lapse imaging of zebrafish embryos. Blocking function of miR-219, a microRNA that down regulates apical Par polarity proteins and promotes progenitor differentiation, elevated Shh signaling. Inhibition of Shh signaling reversed the effects of miR-219 depletion and forced expression of Shh phenocopied miR-219 deficiency. Time-lapse imaging revealed that knockdown of miR-219 function accelerated the growth of primary cilia, revealing a possible mechanistic link between miR-219-mediated regulation of apical Par proteins and Shh signaling. Thus, miR-219 appears to decrease progenitor sensitivity to Shh signaling, thereby driving them toward differentiation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes