PUBLICATION
A clinically relevant in vivo zebrafish model of human multiple myeloma (MM) to study preclinical therapeutic efficacy
- Authors
- Lin, J., Zhang, W., Zhao, J.J., Kwart, A.H., Yang, C., Ma, D., Ren, X., Tai, Y.T., Anderson, K.C., Handin, R.I., Munshi, N.C.
- ID
- ZDB-PUB-160522-5
- Date
- 2016
- Source
- Blood 128(2): 249-52 (Journal)
- Registered Authors
- Handin, Robert, Ma, Dongdong
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Heterografts
- Humans
- Multiple Myeloma/metabolism*
- Multiple Myeloma/pathology*
- Neoplasm Transplantation
- Neoplasms, Experimental/metabolism*
- Neoplasms, Experimental/pathology*
- Zebrafish/metabolism*
- PubMed
- 27207793 Full text @ Blood
Citation
Lin, J., Zhang, W., Zhao, J.J., Kwart, A.H., Yang, C., Ma, D., Ren, X., Tai, Y.T., Anderson, K.C., Handin, R.I., Munshi, N.C. (2016) A clinically relevant in vivo zebrafish model of human multiple myeloma (MM) to study preclinical therapeutic efficacy. Blood. 128(2):249-52.
Abstract
Patient-derived multiple myeloma (MM) cells are difficult to establish in culture or propagate in vivo in murine model. Here, we describe a zebrafish xenograft model that permits rapid, reliable growth of human MM cells injected into the perivitelline space of albino zebrafish (Casper) embryos, 48 hours post-fertilization. MM1S and MM1R MM cell lines and primary CD138(+) MM cells, were stained with CM-Dil red fluorescent dye, suspended in Matrigel prior to their injection. The cells grow at the site of injection and disseminate throughout the developing embryos and larvae. Tumor size was quantified by fluorescent microscopy and cell fate was followed for 4 days. All of the cell line xenografts showed responses similar to those previously observed with in vitro assays. CD138(+) plasma cell xenografts derived from newly-diagnosed MM patients also grew and were inhibited by the same drugs patients had responded to clinically. Using this technique, we can assess drug sensitivity or resistance with a small number of MM cells, in a short period. This raises the possibility that one might be able to assess drug sensitivity in real time with readily obtainable clinical samples.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping