PUBLICATION
The Toolbox for Conditional Zebrafish Cancer Models
- Authors
- Mayrhofer, M., Mione, M.
- ID
- ZDB-PUB-160512-30
- Date
- 2016
- Source
- Advances in experimental medicine and biology 916: 21-59 (Chapter)
- Registered Authors
- Mione, Marina
- Keywords
- Cancer models, Cre/lox, ER/tamoxifen, Gal4/UAS, Heat shock, Inducible systems, Tet-On, Transgenic methods, Zebrafish
- MeSH Terms
-
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats
- Disease Models, Animal*
- Hormones/physiology
- Neoplasms/genetics
- Neoplasms/pathology*
- Neoplasms/physiopathology
- Optogenetics
- Zebrafish
- PubMed
- 27165348 Full text @ Adv. Exp. Med. Biol.
Citation
Mayrhofer, M., Mione, M. (2016) The Toolbox for Conditional Zebrafish Cancer Models. Advances in experimental medicine and biology. 916:21-59.
Abstract
Here we describe the conditional zebrafish cancer toolbox, which allows for fine control of the expression of oncogenes or downregulation of tumor suppressors at the spatial and temporal level. Methods such as the Gal4/UAS or the Cre/lox systems paved the way to the development of elegant tumor models, which are now being used to study cancer cell biology, clonal evolution, identification of cancer stem cells and anti-cancer drug screening. Combination of these tools, as well as novel developments such as the promising genome editing system through CRISPR/Cas9 and clever application of light reactive proteins will enable the development of even more sophisticated zebrafish cancer models. Here, we introduce this growing toolbox of conditional transgenic approaches, discuss its current application in zebrafish cancer models and provide an outlook on future perspectives.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping