ZFIN ID: ZDB-PUB-160512-30
The Toolbox for Conditional Zebrafish Cancer Models
Mayrhofer, M., Mione, M.
Date: 2016
Source: Advances in experimental medicine and biology 916: 21-59 (Chapter)
Registered Authors: Mione, Marina
Keywords: Cancer models, Cre/lox, ER/tamoxifen, Gal4/UAS, Heat shock, Inducible systems, Tet-On, Transgenic methods, Zebrafish
MeSH Terms:
  • Animals
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Disease Models, Animal*
  • Hormones/physiology
  • Neoplasms/genetics
  • Neoplasms/pathology*
  • Neoplasms/physiopathology
  • Optogenetics
  • Zebrafish
PubMed: 27165348 Full text @ Adv. Exp. Med. Biol.
Here we describe the conditional zebrafish cancer toolbox, which allows for fine control of the expression of oncogenes or downregulation of tumor suppressors at the spatial and temporal level. Methods such as the Gal4/UAS or the Cre/lox systems paved the way to the development of elegant tumor models, which are now being used to study cancer cell biology, clonal evolution, identification of cancer stem cells and anti-cancer drug screening. Combination of these tools, as well as novel developments such as the promising genome editing system through CRISPR/Cas9 and clever application of light reactive proteins will enable the development of even more sophisticated zebrafish cancer models. Here, we introduce this growing toolbox of conditional transgenic approaches, discuss its current application in zebrafish cancer models and provide an outlook on future perspectives.