ZFIN ID: ZDB-PUB-160512-26
Genomic Approaches to Zebrafish Cancer
White, R.M.
Date: 2016
Source: Advances in experimental medicine and biology   916: 125-45 (Chapter)
Registered Authors: White, Richard M.
Keywords: Cancer, Next-generation sequencing, Oncogenomics, RNA-seq, Zebrafish
MeSH Terms:
  • Animals
  • Disease Models, Animal*
  • Exome
  • Genomics*
  • High-Throughput Nucleotide Sequencing
  • Neoplasms/genetics*
  • Oligonucleotide Array Sequence Analysis
  • Zebrafish
PubMed: 27165352 Full text @ Adv. Exp. Med. Biol.
The zebrafish has emerged as an important model for studying cancer biology. Identification of DNA, RNA and chromatin abnormalities can give profound insight into the mechanisms of tumorigenesis and the there are many techniques for analyzing the genomes of these tumors. Here, I present an overview of the available technologies for analyzing tumor genomes in the zebrafish, including array based methods as well as next-generation sequencing technologies. I also discuss the ways in which zebrafish tumor genomes can be compared to human genomes using cross-species oncogenomics, which act to filter genomic noise and ultimately uncover central drivers of malignancy. Finally, I discuss downstream analytic tools, including network analysis, that can help to organize the alterations into coherent biological frameworks that can then be investigated further.