PUBLICATION
In vivo physiological recording from the lateral line of juvenile zebrafish
- Authors
- Olt, J., Allen, C.E., Marcotti, W.
- ID
- ZDB-PUB-160511-5
- Date
- 2016
- Source
- The Journal of physiology 594(19): 5427-38 (Journal)
- Registered Authors
- Allen, Claire
- Keywords
- none
- MeSH Terms
-
- Anesthetics, Local/pharmacology
- Animals
- Benzocaine/pharmacology
- Electrophysiological Phenomena
- Lateral Line System/physiology*
- Mice
- Sensory Receptor Cells/physiology*
- Zebrafish/physiology*
- PubMed
- 27161862 Full text @ J. Physiol.
Citation
Olt, J., Allen, C.E., Marcotti, W. (2016) In vivo physiological recording from the lateral line of juvenile zebrafish. The Journal of physiology. 594(19):5427-38.
Abstract
Hair cells are sensory receptors responsible for transducing auditory and vestibular information into electrical signals, which are then transmitted with remarkable precision to afferent neurons. The zebrafish lateral line is emerging as an excellent in vivo model for genetic and physiological analysis of hair cells and neurons. However, research has been limited to larval stages because zebrafish become protected from the time of independent feeding under European law (from 5.2 days post-fertilization, dpf, at 28.5°C). In larval zebrafish, the functional properties of hair cells, as well as those of other excitable cells, are still immature. We have developed an experimental protocol to record electrophysiological properties from hair cells of the lateral line in juvenile zebrafish. We found that the anaesthetic benzocaine at 50 mg l(-1) was an effective and safe anaesthetic to use on juvenile zebrafish. Concentrations up to 300 mg l(-1) did not affect the electrical properties or synaptic vesicle release of juvenile hair cells, unlike the commonly used anaesthetic MS-222, which reduces the size of basolateral membrane currents. Additionally, we implemented a method to maintain gill movement, and as such respiration and blood oxygenation, via the intubation of > 21 dpf zebrafish. The combination of benzocaine and intubation provides an experimental platform to investigate the physiology of mature hair cells from live zebrafish. More generally, this method would allow functional studies involving live imaging and electrophysiology from juvenile and adult zebrafish. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping