PUBLICATION
Combined toxicity of silica nanoparticles and methylmercury on cardiovascular system in zebrafish (Danio rerio) embryos
- Authors
- Duan, J., Hu, H., Li, Q., Jiang, L., Zou, Y., Wang, Y., Sun, Z.
- ID
- ZDB-PUB-160511-2
- Date
- 2016
- Source
- Environmental Toxicology and Pharmacology 44: 120-127 (Journal)
- Registered Authors
- Keywords
- Cardiovascular system, Combined toxicity, Methylmercury, Silica nanoparticles, Zebrafish embryos
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cardiovascular System/drug effects*
- Drug Synergism
- Embryo, Nonmammalian
- Erythrocytes/drug effects
- Methylmercury Compounds/toxicity*
- Nanoparticles/toxicity*
- No-Observed-Adverse-Effect Level
- Reactive Oxygen Species/metabolism
- Silicon Dioxide/toxicity*
- Zebrafish
- PubMed
- 27163730 Full text @ Environ. Toxicol. Pharmacol.
Citation
Duan, J., Hu, H., Li, Q., Jiang, L., Zou, Y., Wang, Y., Sun, Z. (2016) Combined toxicity of silica nanoparticles and methylmercury on cardiovascular system in zebrafish (Danio rerio) embryos. Environmental Toxicology and Pharmacology. 44:120-127.
Abstract
This study was to investigate the combined toxicity of silica nanoparticles (SiNPs) and methylmercury (MeHg) on cardiovascular system in zebrafish (Danio rerio) embryos. Ultraviolet absorption analysis showed that the co-exposure system had high absorption and stability. The dosages used in this study were based on the NOAEL level. Zebrafish embryos exposed to the co-exposure of SiNPs and MeHg did not show any cardiovascular malformation or atrioventricular block, but had an inhibition effect on bradycardia. Using o-Dianisidine for erythrocyte staining, the cardiac output of zebrafish embryos was decreased gradually in SiNPs, MeHg, co-exposure groups, respectively. Co-exposure of SiNPs and MeHg enhanced the vascular endothelial damage in Tg(fli-1:EGFP) transgenic zebrafish line. Moreover, the co-exposure significantly activated the oxidative stress and inflammatory response in neutrophils-specific Tg(mpo:GFP) transgenic zebrafish line. This study suggested that the combined toxic effects of SiNPs and MeHg on cardiovascular system had more severe toxicity than the single exposure alone.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping