ZFIN ID: ZDB-PUB-160507-8
RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish
Wei, Y., Xu, J., Zhang, W., Wen, Z., Liu, F.
Date: 2016
Source: Development (Cambridge, England)   143(12): 2103-10 (Journal)
Registered Authors: Liu, Feng, Wei, Yonglong, Wen, Zilong
Keywords: RNA polymerase III, Rpc9, P53, Hematopoietic stem and progenitor cells, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Proliferation
  • Cell Survival
  • Embryo, Nonmammalian/metabolism
  • Gene Knockdown Techniques
  • Hematopoiesis
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism*
  • Mutation/genetics
  • Phenotype
  • RNA Polymerase III/metabolism*
  • T-Lymphocytes/cytology
  • Tumor Suppressor Protein p53/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/metabolism*
PubMed: 27151951 Full text @ Development
Hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and replenishing all lineages of blood cells throughout the lifetime and thus critical for tissue homeostasis. However, the mechanism regulating HSPC development is still incompletely understood. Here, we isolate a zebrafish mutant with defective T lymphopoiesis and positional cloning identifies that Rpc9, a component of DNA-directed RNA polymerase III (Pol III) complex, is responsible for the mutant phenotype. Further analysis shows that rpc9-deficiency leads to the impairment of HSPCs and their derivatives in zebrafish embryos. Excessive apoptosis is observed in the caudal hematopoietic tissue (CHT, the equivalent of fetal liver in mammals) of rpc9(-/-) embryos and the hematopoietic defects in rpc9(-/-) embryos can be fully rescued by suppression of p53 Thus, our work illustrate that Rpc9, a component of Pol III, plays an important tissue-specific role in HSPC maintenance during zebrafish embryogenesis and that it might be conserved across vertebrates including mammals.