PUBLICATION

A novel cold-sensitive mutant of ntla reveals temporal roles of Brachyury in zebrafish

Authors
Kimelman, D.
ID
ZDB-PUB-160507-3
Date
2016
Source
Developmental dynamics : an official publication of the American Association of Anatomists   245(8): 874-80 (Journal)
Registered Authors
Kimelman, David
Keywords
Brachyury, T-box genes, early vertebrate development, neuromesodermal progenitors
MeSH Terms
  • Animals
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Cold Temperature
  • Fetal Proteins/genetics
  • Fetal Proteins/metabolism*
  • Fluorescent Antibody Technique
  • Gastrula/embryology
  • Gastrula/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • In Situ Hybridization
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27153483 Full text @ Dev. Dyn.
Abstract
With the exception of the head, the vertebrate embryonic body is formed progressively in an anterior-posterior direction, originating from a posteriorly located bipotential neural-mesodermal progenitor population. The T-box transcription factor Brachyury is expressed within the progenitors, and is essential for the formation of the posterior mesoderm. A novel cold-sensitive mutant of zebrafish Brachyury (ntla(cs) ) is described that allows exploration of the temporal role of this key factor.
The ntla(cs) mutant is used to show that Ntla has an essential role during early gastrulation, but as gastrulation proceeds the importance of Ntla declines as Ntlb acquires a capacity to form the posterior mesoderm. Remarkably, ntla(cs) embryos held at the non-permissive temperature just during the gastrula stages show recovery of normal levels of mesodermal gene expression, demonstrating the plasticity of the posterior progenitors.
ntla(cs) is a valuable tool for exploring the processes forming the posterior body since it allows temporally specific activation and inactivation of Brachyury function. It is used here to show the changing roles of Ntla during early development, and the dynamics of the neuromesdermal progenitors. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes