PUBLICATION
Thyroid hormone disruption by water-accommodated fractions of crude oil and sediments affected by the Hebei Spirit oil spill in zebrafish and GH3 cells
- Authors
- Kim, S., Sohn, J.H., Ha, S.Y., Kang, H., Yim, U.H., Shim, W.J., Khim, J.S., Jung, D., Choi, K.
- ID
- ZDB-PUB-160505-2
- Date
- 2016
- Source
- Environmental science & technology 50(11): 5972-80 (Journal)
- Registered Authors
- Choi, Kyungho
- Keywords
- none
- MeSH Terms
-
- Animals
- Iran
- Petroleum*
- Petroleum Pollution*
- Thyroid Hormones
- Water
- Zebrafish
- PubMed
- 27144452 Full text @ Env. Sci. Tech.
Citation
Kim, S., Sohn, J.H., Ha, S.Y., Kang, H., Yim, U.H., Shim, W.J., Khim, J.S., Jung, D., Choi, K. (2016) Thyroid hormone disruption by water-accommodated fractions of crude oil and sediments affected by the Hebei Spirit oil spill in zebrafish and GH3 cells. Environmental science & technology. 50(11):5972-80.
Abstract
A crude oil and the coastal sediments that were affected by Hebei Spirit Oil Spill (HSOS) of Taean, Korea were investigated for thyroid hormone disruption potentials. Water-accommodated fractions (WAFs) of Iranian Heavy crude oil, the major oil type of HSOS, and the porewater or leachate of sediment samples collected along the coast line of Taean were tested for thyroid disruption using developing zebrafish, and/or rat pituitary GH3 cells. Major polycyclic aromatic hydrocarbons (PAHs) and their alkylated forms were also measured from the test samples. In zebrafish larvae, significant decreases in whole-body thyroxine (T4) and triiodothyronine (T3) levels, along with transcriptional changes of thyroid regulating genes, were observed following 5 day exposure to WAFs. In GH3 cells, transcriptions of thyroid regulating genes were influenced following the exposure to the sediment samples, but the pattern of the regulatory change was different from those observed from the WAFs. Composition of PAHs and their alkylated homologs in the WAFs could partly explain this difference. Our results clearly demonstrate that WAFs of crude oil can disrupt thyroid function of larval zebrafish. Sediment samples also showed thyroid disrupting potentials in the GH3 cell, even several years after the oil spill. Long-term ecosystem consequences of thyroid hormone disruption due to oil spill deserve further investigation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping