PUBLICATION
Steroid androgen 17α-methyltestosterone induces malformations and biochemical alterations in zebrafish embryos
- Authors
- Rivero-Wendt, C.L., Oliveira, R., Monteiro, M.S., Domingues, I., Soares, A.M., Grisolia, C.K.
- ID
- ZDB-PUB-160504-10
- Date
- 2016
- Source
- Environmental Toxicology and Pharmacology 44: 107-113 (Journal)
- Registered Authors
- Soares, Amadeu
- Keywords
- Biomarkers, Endocrine disruptor, Fish embryo test, Oxidative stress, Vitellogenin
- MeSH Terms
-
- Animals
- Aquaculture
- Catalase/metabolism
- Cholinesterases/metabolism
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Female
- Fish Proteins/metabolism
- Glutathione Transferase/metabolism
- L-Lactate Dehydrogenase/metabolism
- Methyltestosterone/toxicity*
- Vitellogenins/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 27137108 Full text @ Environ. Toxicol. Pharmacol.
Citation
Rivero-Wendt, C.L., Oliveira, R., Monteiro, M.S., Domingues, I., Soares, A.M., Grisolia, C.K. (2016) Steroid androgen 17α-methyltestosterone induces malformations and biochemical alterations in zebrafish embryos. Environmental Toxicology and Pharmacology. 44:107-113.
Abstract
The synthetic androgen 17α-methyltestosterone is widely used in fish aquaculture for sex reversion of female individuals. Little is known about the amount of MT residues reaching the aquatic environment and further impacts in non-target organisms, including fish early-life stages. Thus, in this work, zebrafish embryos were exposed to two forms of 17α-methyltestosterone: the pure compound (MT) and a formulation commonly used in Brazil (cMT). For MT, a 96h-LC50 of 10.09mg/l was calculated. MT also affected embryo development inducing tail malformations, edemas, abnormal development of the head, and hatching delay. At biochemical level MT inhibited vitellogenin (VTG) and inhibited cholinesterase and lactate dehydrogenase. cMT elicited similar patterns of toxicity as the pure compound (MT). Effects reported in this study suggest a potential environmental risk of MT, especially since the VTG effects occurred at environmental relevant concentrations (0.004mg/l).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping