PUBLICATION

Bmp signaling mediates endoderm pouch morphogenesis by regulating Fgf signaling in zebrafish

Authors
Lovely, C.B., Swartz, M.E., McCarthy, N., Norrie, J.L., Eberhart, J.K.
ID
ZDB-PUB-160429-8
Date
2016
Source
Development (Cambridge, England)   143(11): 2000-11 (Journal)
Registered Authors
Eberhart, Johann, McCarthy, Neil, Swartz, Mary
Keywords
Bmp signaling, Pharyngeal pouches, Endoderm morphogenesis, FGF signaling, Zebrafish
MeSH Terms
  • Animals
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Count
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Endoderm/drug effects
  • Endoderm/embryology*
  • Endoderm/metabolism
  • Face/embryology
  • Fibroblast Growth Factors/metabolism*
  • Morphogenesis*/drug effects
  • Neural Crest/drug effects
  • Neural Crest/metabolism
  • Neural Crest/pathology
  • Pyrazoles/pharmacology
  • Pyrimidines/pharmacology
  • Signal Transduction*/drug effects
  • Skull/drug effects
  • Skull/pathology
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
PubMed
27122171 Full text @ Development
Abstract
The endodermal pouches are a series of reiterated structures that segment the pharyngeal arches and help pattern the vertebrate face. Multiple pathways regulate the complex process of endodermal development including the Bone Morphogenetic Protein (Bmp) pathway. However, the role of Bmp signaling in pouch morphogenesis is poorly understood. Using genetic and chemical inhibitor approaches, we show that pouch morphogenesis requires Bmp signaling from 10-18 hours post-fertilization, immediately following gastrulation. Blocking Bmp signaling during this window results in morphological defects to the pouches and craniofacial skeleton. Using genetic chimeras we show that Bmp signals directly to the endoderm for proper morphogenesis. Time-lapse imaging and analysis of reporter transgenics show that Bmp signaling is necessary for pouch outpocketing via the Fibroblast Growth Factor (Fgf) pathway. Double loss-of-function analyses demonstrate that Bmp and Fgf signaling interact synergistically in craniofacial development. Collectively, our analyses shed light on the tissue and signaling interactions regulating development of the vertebrate face.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes