ZFIN ID: ZDB-PUB-160426-2
Triclosan is a mitochondrial uncoupler in live zebrafish
Shim, J., Weatherly, L.M., Luc, R.H., Dorman, M.T., Neilson, A., Ng, R., Kim, C.H., Millard, P.J., Gosse, J.A.
Date: 2016
Source: Journal of applied toxicology : JAT   36(12): 1662-1667 (Journal)
Registered Authors: Kim, Carol H., Luc, Richard H.
Keywords: ATP production, Extracellular Flux Analyzer, bioenergetics, irgasan, mitochondrial uncoupler, oxygen consumption rate, proton leak, seahorse bioscience, triclosan, zebrafish
MeSH Terms:
  • Animals
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian/drug effects*
  • Environmental Pollutants/toxicity*
  • Mitochondria/drug effects*
  • Mitochondria/metabolism
  • Oxygen Consumption/drug effects
  • Protons
  • Triclosan/toxicity*
  • Uncoupling Agents/toxicity*
  • Zebrafish*/embryology
  • Zebrafish*/metabolism
PubMed: 27111768 Full text @ J. Appl. Toxicol.
ABSTRACT
Triclosan (TCS) is a synthetic antimicrobial agent used in many consumer goods at millimolar concentrations. As a result of exposure, TCS has been detected widely in humans. We have recently discovered that TCS is a proton ionophore mitochondrial uncoupler in multiple types of living cells. Here, we present novel data indicating that TCS is also a mitochondrial uncoupler in a living organism: 24-hour post-fertilization (hpf) zebrafish embryos. These experiments were conducted using a Seahorse Bioscience XF(e) 96 Extracellular Flux Analyzer modified for bidirectional temperature control, using the XF96 spheroid plate to position and measure one zebrafish embryo per well. Using this method, after acute exposure to TCS, the basal oxygen consumption rate (OCR) increases, without a decrease in survival or heartbeat rate. TCS also decreases ATP-linked respiration and spare respiratory capacity and increases proton leak: all indicators of mitochondrial uncoupling. Our data indicate, that TCS is a mitochondrial uncoupler in vivo, which should be taken into consideration when assessing the toxicity and/or pharmaceutical uses of TCS. This is the first example of usage of a Seahorse Extracellular Flux Analyzer to measure bioenergetic flux of a single zebrafish embryo per well in a 96-well assay format. The method developed in this study provides a high-throughput tool to identify previously unknown mitochondrial uncouplers in a living organism.
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