ZFIN ID: ZDB-PUB-160420-9
Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes
Garcia-Reyero, N., Escalon, L., Prats, E., Faria, M., Soares, A.M., Raldúa, D.
Date: 2016
Source: Bulletin of environmental contamination and toxicology   96(6): 707-713 (Journal)
Registered Authors: Raldúa, Demetrio, Soares, Amadeu
Keywords: Chlorpyrifos-oxon, Gene expression, Mitochondria, Oxidative stress, Zebrafish
MeSH Terms:
  • Animals
  • Chlorpyrifos/analogs & derivatives*
  • Chlorpyrifos/toxicity
  • Gene Expression/drug effects*
  • Lipid Peroxidation/drug effects
  • Oxidative Stress/drug effects*
  • Phenotype
  • Zebrafish/metabolism
PubMed: 27086301 Full text @ Bull. Environ. Contam. Toxicol.
Zebrafish models for mild, moderate, and severe acute organophosphorus poisoning were previously developed by exposing zebrafish larvae to chlopyrifos-oxon. The phenotype of these models was characterized at several levels of biological organization. Oxidative stress and mitochondrial dysfunction were found to be involved in the development of the more severe phenotype. Here we used targeted gene expression to understand the dose-responsiveness of those two pathways and their involvement on generating the different zebrafish models. As the severe phenotype is irreversible after only 3 h of exposure, we also analyzed the response of the oxidative stress pathway at 3 and 24 h. Some of the genes related to oxidative stress were already differentially expressed at 3 h. There was an increase in differentially expressed genes related to both oxidative stress and mitochondrial function from the more mild to the more severe phenotype, suggesting the involvement of these mechanisms in increasing phenotype severity. Temporal data suggest that peroxynitrite leading to lipid peroxidation might be involved in phenotype transition and irreversibility.