PUBLICATION

Dense EM-based reconstruction of the interglomerular projectome in the zebrafish olfactory bulb

Authors
Wanner, A.A., Genoud, C., Masudi, T., Siksou, L., Friedrich, R.W.
ID
ZDB-PUB-160420-6
Date
2016
Source
Nature Neuroscience   19: 816-825 (Journal)
Registered Authors
Friedrich, Rainer
Keywords
Neural circuits, Olfactory bulb, Scanning electron microscopy
MeSH Terms
  • Animals
  • Interneurons/ultrastructure*
  • Larva/ultrastructure
  • Microscopy, Electron/methods
  • Nerve Net/metabolism
  • Olfactory Bulb/ultrastructure*
  • Sensory Receptor Cells/ultrastructure*
  • Synapses/metabolism*
  • Zebrafish
PubMed
27089019 Full text @ Nat. Neurosci.
Abstract
The dense reconstruction of neuronal circuits from volumetric electron microscopy (EM) data has the potential to uncover fundamental structure-function relationships in the brain. To address bottlenecks in the workflow of this emerging methodology, we developed a procedure for conductive sample embedding and a pipeline for neuron reconstruction. We reconstructed ∼98% of all neurons (>1,000) in the olfactory bulb of a zebrafish larva with high accuracy and annotated all synapses on subsets of neurons representing different types. The organization of the larval olfactory bulb showed marked differences from that of the adult but similarities to that of the insect antennal lobe. Interneurons comprised multiple types but granule cells were rare. Interglomerular projections of interneurons were complex and bidirectional. Projections were not random but biased toward glomerular groups receiving input from common types of sensory neurons. Hence, the interneuron network in the olfactory bulb exhibits a specific topological organization that is governed by glomerular identity.
Genes / Markers
Figures
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
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Orthology
Engineered Foreign Genes
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