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ZFIN ID: ZDB-PUB-160417-4
Protective effect of a recombinant VHSV-G vaccine using poly(I:C) loaded nanoparticles as an adjuvant in zebrafish (Danio rerio) infection model
Kavaliauskis, A., Arnemo, M., Speth, M.T., Lagos, L., Rishovd, A.L., Estepa, A., Griffiths, G., Gjøen, T.
Date: 2016
Source: Developmental and comparative immunology   61: 248-57 (Journal)
Registered Authors:
Keywords: Adjuvant, Nanoparticles, Poly(I:C), VHSV vaccine, Zebrafish
MeSH Terms:
  • Adjuvants, Immunologic/administration & dosage*
  • Animals
  • Aquaculture
  • Cells, Cultured
  • Chitosan/chemistry
  • Chitosan/immunology
  • Hemorrhagic Septicemia, Viral/immunology*
  • Hemorrhagic Septicemia, Viral/prevention & control
  • Immunity
  • Leukocytes/immunology
  • Nanoparticles/administration & dosage*
  • Nanoparticles/chemistry
  • Novirhabdovirus/immunology*
  • Poly I-C/chemistry
  • Poly I-C/immunology*
  • Vaccines, Synthetic
  • Viral Vaccines/immunology*
  • Zebrafish/immunology*
PubMed: 27084059 Full text @ Dev. Comp. Immunol.
There is a constant need to increase the efficiency of vaccines in the aquaculture industry. Although several nano-based vaccine formulations have been reported, to the best of our knowledge so far only one of them have been implemented in the industry. Here we report on chitosan-poly(I:C) nanoparticles (NPs) that could be used as a non-specific adjuvant in antiviral vaccines in aquaculture. We have characterized the physical parameters of the NPs, studied the in vivo and in vitro bio-distribution of fluorescent NPs and verified NP uptake by zebrafish leucocytes. We used the zebrafish model to test the protective efficiency of the recombinant glycoprotein G (rgpG) of VHSV compared to inactivated whole virus (iV) against VHSV using NPs as an adjuvant in both formulations. In parallel we tested free poly(I:C) and rgpG (pICrgpG), and free chitosan and rgpG (CSrgpG) vaccine formulations. While the iV group (with NP adjuvant) provided the highest overall survival, all vaccine formulations with poly(I:C) provided a significant protection against VHSV; possibly through an early induction of an anti-viral state. Our results suggest that chitosan-poly(I:C) NPs are a promising adjuvant candidate for future vaccine formulations.