PUBLICATION
Optical micromanipulation of nanoparticles and cells inside living zebrafish
- Authors
- Johansen, P.L., Fenaroli, F., Evensen, L., Griffiths, G., Koster, G.
- ID
- ZDB-PUB-160322-8
- Date
- 2016
- Source
- Nature communications 7: 10974 (Journal)
- Registered Authors
- Evensen, Lasse
- Keywords
- Biological sciences, Biophysics, Nanotechnology
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Erythrocytes/metabolism
- Macrophages/metabolism
- Microinjections
- Micromanipulation/methods*
- Nanoparticles/chemistry*
- Nanotubes/chemistry
- Optical Tweezers*
- Zebrafish/metabolism*
- PubMed
- 26996121 Full text @ Nat. Commun.
Citation
Johansen, P.L., Fenaroli, F., Evensen, L., Griffiths, G., Koster, G. (2016) Optical micromanipulation of nanoparticles and cells inside living zebrafish. Nature communications. 7:10974.
Abstract
Regulation of biological processes is often based on physical interactions between cells and their microenvironment. To unravel how and where interactions occur, micromanipulation methods can be used that offer high-precision control over the duration, position and magnitude of interactions. However, lacking an in vivo system, micromanipulation has generally been done with cells in vitro, which may not reflect the complex in vivo situation inside multicellular organisms. Here using optical tweezers we demonstrate micromanipulation throughout the transparent zebrafish embryo. We show that different cells, as well as injected nanoparticles and bacteria can be trapped and that adhesion properties and membrane deformation of endothelium and macrophages can be analysed. This non-invasive micromanipulation inside a whole-organism gives direct insights into cell interactions that are not accessible using existing approaches. Potential applications include screening of nanoparticle-cell interactions for cancer therapy or tissue invasion studies in cancer and infection biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping