PUBLICATION
Exercise quantity-dependent muscle hypertrophy in adult zebrafish (Danio rerio)
- Authors
- Hasumura, T., Meguro, S.
- ID
- ZDB-PUB-160310-17
- Date
- 2016
- Source
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 186(5): 603-14 (Journal)
- Registered Authors
- Keywords
- Forced exercise, Muscle hypertrophy, Muscle mass, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression
- Hypertrophy
- Male
- Muscle, Skeletal/pathology
- Muscle, Skeletal/physiology*
- Physical Conditioning, Animal/physiology*
- RNA, Messenger/metabolism
- Swimming/physiology*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- PubMed
- 26951149 Full text @ J. Comp. Physiol. B
Citation
Hasumura, T., Meguro, S. (2016) Exercise quantity-dependent muscle hypertrophy in adult zebrafish (Danio rerio). Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 186(5):603-14.
Abstract
Exercise is very important for maintaining and increasing skeletal muscle mass, and is particularly important to prevent and care for sarcopenia and muscle disuse atrophy. However, the dose-response relationship between exercise quantity, duration/day, and overall duration and muscle mass is poorly understood. Therefore, we investigated the effect of exercise duration on skeletal muscle to reveal the relationship between exercise quantity and muscle hypertrophy in zebrafish forced to exercise. Adult male zebrafish were exercised 6 h/day for 4 weeks, 6 h/day for 2 weeks, or 3 h/day for 2 weeks. Flow velocity was adjusted to maximum velocity during continual swimming (initial 43 cm/s). High-speed consecutive photographs revealed that zebrafish mainly drove the caudal part. Additionally, X-ray micro computed tomography measurements indicated muscle hypertrophy of the mid-caudal half compared with the mid-cranial half part. The cross-sectional analysis of the mid-caudal half muscle revealed that skeletal muscle (red, white, or total) mass increased with increasing exercise quantity, whereas that of white muscle and total muscle increased only under the maximum exercise load condition of 6 h/day for 4 weeks. Additionally, the muscle fiver size distributions of exercised fish were larger than those from non-exercised fish. We revealed that exercise quantity, duration/day, and overall duration were correlated with skeletal muscle hypertrophy. The forced exercise model enabled us to investigate the relationship between exercise quantity and skeletal muscle mass. These results open up the possibility for further investigations on the effects of exercise on skeletal muscle in adult zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping