PUBLICATION
Oxidative stress and immunotoxicity induced by graphene oxide in zebrafish
- Authors
- Chen, M., Yin, J., Liang, Y., Yuan, S., Wang, F., Song, M., Wang, H.
- ID
- ZDB-PUB-160228-1
- Date
- 2016
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 174: 54-60 (Journal)
- Registered Authors
- Keywords
- Acute toxicity, Graphene oxide, Immunotoxicity, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Cytokines/genetics
- Gene Expression Regulation/drug effects*
- Graphite/toxicity*
- Intestines/drug effects
- Liver/drug effects
- Oxidative Stress/drug effects*
- Oxides/toxicity*
- Spleen/drug effects
- Water Pollutants, Chemical/toxicity
- Zebrafish/genetics
- Zebrafish/immunology
- Zebrafish/physiology*
- PubMed
- 26921726 Full text @ Aquat. Toxicol.
Citation
Chen, M., Yin, J., Liang, Y., Yuan, S., Wang, F., Song, M., Wang, H. (2016) Oxidative stress and immunotoxicity induced by graphene oxide in zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 174:54-60.
Abstract
Graphene oxide (GO) has been extensively explored as a promising nanomaterial for applications in biology because of its unique properties. Therefore, systematic investigation of GO toxicity is essential to determine its fate in the environment and potential adverse effects. In this study, acute toxicity, oxidative stress and immunotoxicity of GO were investigated in zebrafish. No obvious acute toxicity was observed when zebrafish were exposed to 1, 5, 10 or 50mg/L GO for 14 days. However, a number of cellular alterations were detected by histological analysis of the liver and intestine, including vacuolation, loose arrangement of cells, histolysis and disintegration of cell boundaries. As evidence for oxidative stress, malondialdehyde levels and superoxide dismutase and catalase activities were increased and glutathione content was decreased in the liver after treatment with GO. GO treatment induced an immune response in zebrafish, as demonstrated by increased expression of tumor necrosis factor α, interleukin-1 β, and interleukin-6 in the spleen. Our findings demonstrated that GO administration in an aquatic system can cause oxidative stress and immune toxicity in adult zebrafish. To our knowledge, this is the first report of immune toxicity of GO in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping