PUBLICATION
High-throughput methods for visualizing the teleost skeleton: capturing autofluorescence of alizarin red
- Authors
- Yelick, P. C., Connolly, M. H.
- ID
- ZDB-PUB-160219-18
- Date
- 2010
- Source
- Zeitschrift fur angewandte Ichthyologie = Journal of applied ichthyology 26(2): 274-277 (Journal)
- Registered Authors
- Connolly, Michelle, Yelick, Pamela C.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none Full text @ Zeitschrift Angew. Ichthyol. (J. Appl. Ichtyol.)
Citation
Yelick, P. C., Connolly, M. H. (2010) High-throughput methods for visualizing the teleost skeleton: capturing autofluorescence of alizarin red. Zeitschrift fur angewandte Ichthyologie = Journal of applied ichthyology. 26(2):274-277.
Abstract
For nearly a century, Alizarin red S (alizarin sodium sulfonate) has been used by morphologists to stain calcified bone matrix. More recently, its traditional use has frequently been replaced by more modern techniques; however, its auto fluorescent property continues to contribute to research well beyond the context of bone development and regeneration. The purpose of this study is to describe detailed methods that can be used to capture the autofluorescence of Alizarin red-stained mineralized tissues in juvenile zebrafish. These methods allow for in situ analyses of minute skeletal elements, such as pharyngeal teeth, and preclude the need for dissection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping