ZFIN ID: ZDB-PUB-160216-16
Grass carp Ctenopharyngodon idella Fibulin-4 as a potential interacting partner for grass carp reovirus outer capsid proteins
Yu, F., Wang, H., Liu, W., Lu, L.
Date: 2016
Source: Fish & shellfish immunology   48: 169-74 (Journal)
Registered Authors: Wang, Hao
Keywords: Extracellular matrix protein, Fibulin-4, Grass carp Ctenopharyngodon idella, Grass carp reovirus, Protein–protein interaction
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/metabolism
  • Capsid Proteins/genetics
  • Capsid Proteins/metabolism*
  • Carps/genetics
  • Carps/metabolism*
  • DNA, Complementary/genetics
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism*
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Intestines/metabolism
  • Molecular Sequence Data
  • Muscles/metabolism
  • Reoviridae
PubMed: 26626583 Full text @ Fish Shellfish Immunol.
Mammalian EGF containing fibulin-like extracellular matrix protein 2 (Fibulin-4/EFEMP2), an extracellular matrix(ECM) protein and a member of the fibulin family, is involved in elastic fiber formation, connective tissue development and some human diseases. In a yeast-two hybrid screening of host proteins interacting with outer capsid protein of grass carp reovirus (GCRV), a grass carp homologue of Fibulin-4 (designated as GcFibulin-4) is suggested to hold the potential to bind VP7, VP56 and VP55, the outer capsid protein encoded by type I, II, III GCRV, respectively. GcFibulin-4 gene of grass carp was cloned and sequenced from the cDNA library constructed for the yeast two-hybrid screening. Full-length cDNA of GcFibulin-4 contains an open reading frame (ORF) of 1323 bp encoding a putative protein of 440 amino acids. Phylogenetic analysis of GcFibulin-4 indicated that it shared a high homology with zebra fish Fibulin-4 protein. Transcriptional distribution analysis of GcFibulin-4 in various tissues of healthy grass carp showed that GcFibulin-4 was highly expressed in muscle, moderately expressed in the intestine and brain, and slightly expressed in other examined tissues; the expression pattern is consistent with tissue tropism of GCRV resulting in hemorrhage symptom in the corresponding tissues. Our results suggested that Fibulin-4 might enable free GCRV particles, the pathogen for grass carp hemorrhagic disease, to target fish tissues more efficiently by interacting with viral outer capsid proteins.