ZFIN ID: ZDB-PUB-160213-17
A novel subset of enteric neurons revealed by ptf1a:GFP in the developing zebrafish enteric nervous system
Uribe, R.A., Gu, T., Bronner, M.E.
Date: 2016
Source: Genesis (New York, N.Y. : 2000)   54(3): 123-8 (Journal)
Registered Authors: Bronner-Fraser, Marianne, Uribe, Rosa
Keywords: enteric nervous system, ptf1a, serotonergic neuron, zebrafish
MeSH Terms:
  • Animals
  • Cell Differentiation
  • Enteric Nervous System/cytology*
  • Enteric Nervous System/embryology
  • Enteric Nervous System/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism*
  • Mice, Transgenic
  • Neural Crest/cytology*
  • Neural Crest/metabolism
  • Neurogenesis
  • Neurons/metabolism*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Serotonin/metabolism
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/growth & development*
PubMed: 26865080 Full text @ Genesis
The enteric nervous system, the largest division of the peripheral nervous system, is derived from vagal neural crest cells that invade and populate the entire length of the gut to form diverse neuronal subtypes. Here, we identify a novel population of neurons within the enteric nervous system of zebrafish larvae that express the transgenic marker ptf1a:GFP within the midgut. Genetic lineage analysis reveals that enteric ptf1a:GFP(+) cells are derived from the neural crest and that most ptf1a:GFP(+) neurons express the neurotransmitter 5HT, demonstrating that they are serotonergic. This transgenic line, Tg(ptf1a:GFP), provides a novel neuronal marker for a subpopulation of neurons within the enteric nervous system, and highlights the possibility that Ptf1a may act as an important transcription factor for enteric neuron development. This article is protected by copyright. All rights reserved.