PUBLICATION
Environmental and cortisol-mediated control of Ca(2+) uptake in tilapia (Oreochromis mossambicus)
- Authors
- Lin, C.H., Kuan, W.C., Liao, B.K., Deng, A.N., Tseng, D.Y., Hwang, P.P.
- ID
- ZDB-PUB-160210-4
- Date
- 2016
- Source
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 186(3): 323-32 (Journal)
- Registered Authors
- Hwang, Pung Pung, Tseng, Deng-Yu
- Keywords
- Ca2+ influx, Cortisol, ECaC, Ionocyte, Tilapia
- MeSH Terms
-
- Acclimatization
- Animals
- Calcium/metabolism*
- Calcium/pharmacology
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gene Expression Regulation
- Gills/metabolism
- Hydrocortisone/metabolism*
- Hydrocortisone/pharmacology
- Ion Transport/drug effects
- Larva/drug effects
- Larva/metabolism
- Plasma Membrane Calcium-Transporting ATPases/genetics
- Plasma Membrane Calcium-Transporting ATPases/metabolism
- Receptors, Glucocorticoid/metabolism
- Receptors, Mineralocorticoid/metabolism
- Sodium-Calcium Exchanger/genetics
- Sodium-Calcium Exchanger/metabolism
- TRPV Cation Channels/genetics
- TRPV Cation Channels/metabolism
- Tilapia/metabolism*
- PubMed
- 26857273 Full text @ J. Comp. Physiol. B
Citation
Lin, C.H., Kuan, W.C., Liao, B.K., Deng, A.N., Tseng, D.Y., Hwang, P.P. (2016) Environmental and cortisol-mediated control of Ca(2+) uptake in tilapia (Oreochromis mossambicus). Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. 186(3):323-32.
Abstract
Ca(2+) is a vital element for many physiological processes in vertebrates, including teleosts, which live in aquatic environments and acquire Ca(2+) from their surroundings. Ionocytes within the adult gills or larval skin are critical sites for transcellular Ca(2+) uptake in teleosts. The ionocytes of zebrafish were found to contain transcellular Ca(2+) transporters, epithelial Ca(2+) channel (ECaC), plasma membrane Ca(2+)-ATPase 2 (PMCA2), and Na(+)/Ca(2+) exchanger 1b (NCX1b), providing information about the molecular mechanism of transcellular Ca(2+) transports mediated by ionocytes in fish. However, more evidence is required to establish whether or not a similar mechanism of transcellular Ca(2+) transport also exists in others teleosts. In the present study, ecac, pmca2, and ncx1 were found to be expressed in the branchial ionocytes of tilapia, thereby providing further support for the mechanism of transcellular Ca(2+) transport through ionocytes previously proposed for zebrafish. In addition, we also reveal that low Ca(2+) water treatment of tilapia stimulates Ca(2+) uptake and expression of ecac and cyp11b (the latter encodes a cortisol-synthesis enzyme). Treatment of tilapia with exogenous cortisol (20 mg/l) enhanced both Ca(2+) influx and ecac expression. Therefore, increased cyp11b expression is suggested to enhance Ca(2+) uptake capacity in tilapia exposed to low Ca(2+) water. Furthermore, the application of cortisol receptor antagonists revealed that cortisol may regulate Ca(2+) uptake through glucocorticoid and/or mineralocorticoid receptor (GR and/or MR) in tilapia. Taken together, the data suggest that cortisol may activate GR and/or MR to execute its hypercalcemic action by stimulating ecac expression in tilapia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping