ZFIN ID: ZDB-PUB-160108-5
Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward
Lawson, N.D.
Date: 2016
Source: Trends in cell biology 26(2): 77-9 (Review)
Registered Authors: Lawson, Nathan
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Gene Knockdown Techniques/methods
  • Gene Knockdown Techniques/trends
  • Humans
  • Morpholinos/genetics
  • Mutagenesis/physiology
  • Mutation/genetics
  • Reverse Genetics*/methods
  • Reverse Genetics*/trends
  • Zebrafish/genetics*
PubMed: 26739910 Full text @ Trends Cell Biol.
Gene editing in zebrafish has begun to reveal discordance between mutant phenotypes and those associated with knockdown via morpholino oligonucleotides (MOs). These studies suggest that MOs should not be used as a standalone tool and underscore the need for guidelines that require defined mutants to assess gene function in zebrafish.