Lawson, N.D. (2016) Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward. Trends in cell biology. 26(2):77-9.
Gene editing in zebrafish has begun to reveal discordance between mutant phenotypes and those associated with knockdown via morpholino oligonucleotides (MOs). These studies suggest that MOs should not be used as a standalone tool and underscore the need for guidelines that require defined mutants to assess gene function in zebrafish.