PUBLICATION

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1

Authors
Zhang, C., Zhang, W., Lu, Y., Yan, X., Yan, X., Zhu, X., Liu, W., Yang, Y., Zhou, T.
ID
ZDB-PUB-151226-2
Date
2016
Source
Cell Research   26(2): 239-53 (Journal)
Registered Authors
Liu, Wei
Keywords
NudC, actin dynamics, ciliogenesis, cofilin 1, zebrafish development
MeSH Terms
  • Actins/metabolism*
  • Animals
  • Cells, Cultured
  • Cilia/metabolism*
  • Cilia/physiology*
  • Cofilin 1/metabolism*
  • HSP90 Heat-Shock Proteins
  • Morphogenesis/physiology*
  • Nuclear Proteins/metabolism*
  • Zebrafish/metabolism
  • Zebrafish/physiology
PubMed
26704451 Full text @ Cell Res.
Abstract
Emerging data indicate that actin dynamics is associated with ciliogenesis. However, the underlying mechanism remains unclear. Here we find that nuclear distribution gene C (NudC), an Hsp90 co-chaperone, is required for actin organization and dynamics. Depletion of NudC promotes cilia elongation and increases the percentage of ciliated cells. Further results show that NudC binds to and stabilizes cofilin 1, a key regulator of actin dynamics. Knockdown of cofilin 1 also facilitates ciliogenesis. Moreover, depletion of either NudC or cofilin 1 causes similar ciliary defects in zebrafish, including curved body, pericardial edema and defective left-right asymmetry. Ectopic expression of cofilin 1 significantly reverses the phenotypes induced by NudC depletion in both cultured cells and zebrafish. Thus, our data suggest that NudC regulates actin cytoskeleton and ciliogenesis by stabilizing cofilin 1.Cell Research advance online publication 25 December 2015; doi:10.1038/cr.2015.152.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes