PUBLICATION
Estimation of the Oxidative Stress and Molecular Damage Caused by 1-Butyl-3-Methylimidazolium Bromide Ionic Liquid in Zebrafish Livers
- Authors
- Dong, M., Liu, T., Wang, J., Wang, J., Zhu, L., Zhang, J.
- ID
- ZDB-PUB-151219-4
- Date
- 2016
- Source
- Journal of biochemical and molecular toxicology 30(5): 232-8 (Journal)
- Registered Authors
- Keywords
- HPLC, MDA, DNA Damage, ROS, SOD
- MeSH Terms
-
- Animals
- Catalase/metabolism
- Comet Assay
- DNA Fragmentation/drug effects*
- Female
- Imidazoles/toxicity*
- Lipid Peroxidation/drug effects
- Liver/drug effects*
- Liver/metabolism
- Liver/pathology
- Male
- Malondialdehyde/metabolism
- Oxidation-Reduction
- Oxidative Stress/drug effects
- Reactive Oxygen Species/metabolism
- Superoxide Dismutase/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism*
- PubMed
- 26681321 Full text @ J. Biochem. Mol. Toxicol.
Citation
Dong, M., Liu, T., Wang, J., Wang, J., Zhu, L., Zhang, J. (2016) Estimation of the Oxidative Stress and Molecular Damage Caused by 1-Butyl-3-Methylimidazolium Bromide Ionic Liquid in Zebrafish Livers. Journal of biochemical and molecular toxicology. 30(5):232-8.
Abstract
The present study investigated the toxic effects of 1-butyl-3-methylimidazolium bromide ([C4 mim]Br) in zebrafish livers after exposure to 5-40 mg/L of [C4 mim]Br on days 7, 14, 21, and 28. The results showed that low levels of [C4 mim]Br exposure activated the superoxide dismutase (SOD) activity during early periods of exposure. However, high concentrations (20-40 mg/L) of [C4 mim]Br caused the inhibition of SOD, which, accordingly, led to excess reactive oxygen species and malondialdehyde. The present results indicate that [C4 mim]Br causes oxidative stress in the livers of both male and female zebrafish. Additionally, the DNA damage revealed that [C4 mim]Br has a genotoxic effect on zebrafish livers, even when the concentration of [C4 mim]Br is low (5 mg/L). The DNA damage was demonstrated by quantifying the DNA strand breakage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping