PUBLICATION
Two highly adhesive lactic acid bacteria (LAB) strains are protective in zebrafish infected with Aeromonas hydrophila by evocation of gut mucosal immunity
- Authors
- Wang, Y., Ren, Z., Fu, L., Su, X.
- ID
- ZDB-PUB-151112-5
- Date
- 2016
- Source
- Journal of applied microbiology 120(2): 441-451 (Journal)
- Registered Authors
- Keywords
- Aeromonas hydrophila, Colonization, Epithelial barrier, Mucosal immunity, Probiotic, Zebrafish
- MeSH Terms
-
- Aeromonas hydrophila/physiology*
- Animals
- Bacterial Adhesion*
- Disease Models, Animal
- Gram-Negative Bacterial Infections/immunology
- Gram-Negative Bacterial Infections/microbiology*
- Humans
- Immunity, Mucosal*
- Interleukin-10/immunology
- Lactic Acid/metabolism
- Lactobacillus plantarum/physiology*
- Probiotics/administration & dosage*
- Tumor Necrosis Factor-alpha/immunology
- Zebrafish/immunology*
- Zebrafish/microbiology
- PubMed
- 26555459 Full text @ J. Appl. Microbiol.
Citation
Wang, Y., Ren, Z., Fu, L., Su, X. (2016) Two highly adhesive lactic acid bacteria (LAB) strains are protective in zebrafish infected with Aeromonas hydrophila by evocation of gut mucosal immunity. Journal of applied microbiology. 120(2):441-451.
Abstract
Aims To increase the knowledge of probiotic effects and potential mechanisms, we report on the use of the zebrafish model to investigate the in vivo colonization ability, as well as the protective effects associated with gut mucosal immune barrier and responses against Aeromonas hydrophila infection of previously characterized probiotic lactic acid bacteria (LAB) strains, Bacillus coagulans 09.712 and Lactobacillus plantarum 08.923, in comparison with that of three commercialized strains.
Methods and results The results indicated differential adhesion capabilities, and B. coagulans and L. plantarum strains exhibited a more robust adhesion capability based on fluorescence observation. Oral delivery of these two strains in zebrafish greatly improved gut epithelium integrity, as well as reduced recruitment and degranulation of mast cells under A. hydrophila challenge. The percentage of intraepithelial lymphocytes (IELs) in probiotic fed groups was significantly higher than those in the control after challenge (p < 0.001). We also found that the abundance of IL-1β mRNA in B. coagulans- and L. plantarum-fed groups remarkably decreased as a function of time of post challenge, and that of TNF-α and IL-10 mRNA was high with the probiotic treatment.
Conclusions Our findings indicate highly adhesive strains of B. coagulans 09.712 and L. plantarum 08.923 have immunoregulatory and immunoprotective roles in effective stimulation of anti-inflammatory response and barrier regeneration within the mucosa to protect zebrafish against infection.
Significance and impact of the study Our work will further support zebrafish as a powerful model to better understand molecular definition of probiotic effects, as well as the probiotic potential of B. coagulans 09.712 and L. plantarum 08.923 of interest to the food industry. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping