PUBLICATION

Temporal and spatial requirements for Nodal-induced anterior mesendoderm and mesoderm in anterior neurulation

Authors
Gonsar, N., Coughlin, A., Clay-Wright, J.A., Borg, B.R., Kindt, L.M., Liang, J.O.
ID
ZDB-PUB-151104-3
Date
2016
Source
Genesis (New York, N.Y. : 2000)   54(1): 3-18 (Journal)
Registered Authors
Liang, Jennifer
Keywords
Nodal, anencephaly, neural tube defects, zebrafish
MeSH Terms
  • Anencephaly
  • Animals
  • Genetic Association Studies
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Mesoderm/metabolism*
  • Neural Tube/metabolism*
  • Neurulation/genetics
  • Nodal Protein/genetics
  • Nodal Protein/metabolism*
  • Notochord/metabolism*
  • Signal Transduction
  • Spatio-Temporal Analysis
  • Zebrafish/embryology*
  • Zebrafish Proteins
PubMed
26528772 Full text @ Genesis
Abstract
Zebrafish with defective Nodal signaling have a phenotype analogous to the fatal human birth defect anencephaly, which is caused by an open anterior neural tube. Previous work in our laboratory found that anterior open neural tube defects in Nodal signaling mutants were caused by defects in mesendodermal/mesodermal tissue. Defects in these mutants are already apparent at neural plate stage, before the neuroepithelium starts to fold into a tube. Consistent with this, we found that the requirement for Nodal signaling maps to mid-late blastula stages. This timing correlates with the timing of prechordal plate mesendoderm and anterior mesoderm induction, suggesting these tissues act to promote neurulation. To further identify tissues important for neurulation, we took advantage of the variable phenotypes in Nodal signaling-deficient sqt mutant and Lefty1-overexpressing embryos. Statistical analysis indicated a strong, positive correlation between a closed neural tube and presence of several mesendoderm/mesoderm-derived tissues (hatching glands, cephalic paraxial mesoderm, notochord, and head muscles). However, the neural tube was closed in a subset of embryos that lacked any one of these tissues. This suggests that several types of Nodal-induced mesendodermal/mesodermal precursors are competent to promote neurulation. This article is protected by copyright. All rights reserved.
Genes / Markers
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes