PUBLICATION
Strain- and context-dependent behavioural responses of acute alarm substance exposure in zebrafish
- Authors
- Quadros, V.A., Silveira, A., Giuliani, G.S., Didonet, F., Silveira, A.S., Nunes, M.E., Silva, T.O., Loro, V.L., Rosemberg, D.B.
- ID
- ZDB-PUB-151103-1
- Date
- 2016
- Source
- Behavioural processes 122: 1-11 (Journal)
- Registered Authors
- Keywords
- Alarm substance, Behaviour, Fear, Strains, Zebrafish
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Anxiety/psychology
- Behavior, Animal/drug effects
- Behavior, Animal/physiology*
- Cues
- Fear/drug effects*
- Fear/psychology
- Female
- Male
- Motor Activity/drug effects
- Motor Activity/physiology*
- Movement/drug effects
- Movement/physiology
- Swimming/physiology
- Zebrafish/physiology*
- PubMed
- 26524408 Full text @ Behav. Processes
Citation
Quadros, V.A., Silveira, A., Giuliani, G.S., Didonet, F., Silveira, A.S., Nunes, M.E., Silva, T.O., Loro, V.L., Rosemberg, D.B. (2016) Strain- and context-dependent behavioural responses of acute alarm substance exposure in zebrafish. Behavioural processes. 122:1-11.
Abstract
We investigate the behavioural responses of wild type (WT) and leopard (leo) zebrafish elicited by alarm substances of conspecifics at three contexts: during the exposure period (Experiment 1); after exposure, in habituation to novelty (Experiment 2); or after exposure, in the light-dark preference test (Experiment 3), and analyse their influence on pigment response. During the exposure, leo showed decreased vertical drifts, increased number and duration of erratic movements, while WT had increased erratic movements and latency to enter the top. In the novel tank, we observed that angular velocity decreased in WT exposed to alarm substance, which also presented increased fear responses. Contrastingly, leo increased the number of entries and time in top, indicating differences in habituation profile. Alarm substance increased the number of erratic movements in the light-dark test, but elicited different responses between strains in scototaxis, latency to enter the dark compartment and risk assessment episodes. Moreover, the body colour of zebrafish did not change after alarm substance exposure. Principal component analyses suggest that burst swimming, anxiety-like behaviours, and locomotion/exploration were the components that most accounted for total variances of Experiments 1, 2, and 3, respectively. We conclude that chemical cue from conspecifics triggers strain- and context-dependent responses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping