PUBLICATION
Genomic structure and promoter functional analysis of GnRH3 gene in large yellow croaker (larimichthys crocea)
- Authors
- Huang, W., Zhang, J., Liao, Z., Lv, Z., Wu, H., Zhu, A., Wu, C.
- ID
- ZDB-PUB-151102-3
- Date
- 2016
- Source
- Gene 576(1 Pt 3): 458-65 (Journal)
- Registered Authors
- Keywords
- Genomic structure, GnRH3, Larimichthys crocea, Promoter analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Base Sequence
- Gonadotropin-Releasing Hormone/chemistry
- Gonadotropin-Releasing Hormone/genetics*
- Molecular Sequence Data
- Open Reading Frames
- Perciformes/genetics*
- Promoter Regions, Genetic*
- Pyrrolidonecarboxylic Acid/analogs & derivatives*
- Pyrrolidonecarboxylic Acid/chemistry
- Sequence Homology, Amino Acid
- PubMed
- 26519998 Full text @ Gene
Citation
Huang, W., Zhang, J., Liao, Z., Lv, Z., Wu, H., Zhu, A., Wu, C. (2016) Genomic structure and promoter functional analysis of GnRH3 gene in large yellow croaker (larimichthys crocea). Gene. 576(1 Pt 3):458-65.
Abstract
Gonadotropin-releasing hormone III (GnRH3) is considered to be a key neurohormone in fish reproduction control. In the present study, the cDNA and genomic sequences of GnRH3 were cloned and characterized from large yellow croaker Larimichthys crocea. The cDNA encoded a protein of 99 amino acids with four functional motifs. The full-length genome sequence was composed of 3797 nucleotides, including four exons and three introns. Higher identities of amino acid sequences and conserved exonintron organizations were found between LcGnRH3 and other GnRH3 genes. In addition, some special features of the sequences were detected in partial species. For example, two specific residues (V and A) were found in the family Sciaenidae, and the unique 75-72bp type of the open reading frame 2 and 3 existed in the family Cyprinidae. Analysis of the 2576bp promoter fragment of LcGnRH3 showed a number of transcription factor binding sites, such as AP1, CREB, GATA-1, HSF, FOXA2, and FOXL1. Promoter functional analysis using an EGFP reporter fusion in zebrafish larvae presented positive signals in the brain, including the olfactory region, the terminal nerve ganglion, the telencephalon, and the hypothalamus. The expression pattern was generally consistent with the endogenous GnRH3 GFP-expressing transgenic zebrafish lines, but the details were different. These resuslts indicate that the structure and function of LcGnRH3 are generally similar to the other teleost GnRH3 genes, but there exist some distinctions among them.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping