PUBLICATION
Effect of combined exposure to lead and decabromodiphenyl ether on neurodevelopment of zebrafish larvae
- Authors
- Zhu, B., Wang, Q., Shi, X., Guo, Y., Xu, T., Zhou, B.
- ID
- ZDB-PUB-151101-1
- Date
- 2016
- Source
- Chemosphere 144: 1646-1654 (Journal)
- Registered Authors
- Guo, YongYong, Zhou, BingSheng
- Keywords
- BDE-209, Co-exposure, Developmental neurotoxicity, Lead, ROS
- MeSH Terms
-
- Animals
- Axons/drug effects*
- Axons/metabolism
- Central Nervous System/drug effects
- Central Nervous System/growth & development
- Central Nervous System/metabolism
- Gene Expression Regulation, Developmental/drug effects*
- Halogenated Diphenyl Ethers/toxicity*
- Lead/toxicity*
- Motor Activity/drug effects
- Motor Neurons/drug effects
- Motor Neurons/metabolism
- Oxidative Stress
- Random Allocation
- Reactive Oxygen Species/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- PubMed
- 26519795 Full text @ Chemosphere
Citation
Zhu, B., Wang, Q., Shi, X., Guo, Y., Xu, T., Zhou, B. (2016) Effect of combined exposure to lead and decabromodiphenyl ether on neurodevelopment of zebrafish larvae. Chemosphere. 144:1646-1654.
Abstract
The effect of combined exposure to decabromodiphenyl ether (BDE-209) and lead (Pb) on neurodevelopment of zebrafish (Danio rerio) larvae was investigated. Zebrafish embryos were exposed to Pb (0, 5, 10, 20 μg/L) and BDE-209 (0, 50, 100, 200 μg/L), either alone or in combination (Mix1: 5 + 50 μg/L, Mix2: 10 + 100 μg/L, Mix3: 20 + 200 μg/L) for up to 144 h post-fertilization. Growth of secondary motoneuron axons and expression of genes related to central nervous system development was significantly inhibited in Mix3 co-exposure group. A significant increase in reactive oxygen species (ROS), lipid peroxidation, DNA damage, and perturbation of the antioxidant system was detected in the Mix3 group compared to single-toxicant treatments or control. Depressed locomotor activity was recorded in the Mix2 and Mix3 groups. Addition of N-acetyl cysteine to Mix3 eliminated excessive ROS, and protected against lipid peroxidation, DNA damage, and locomotor dysfunction. Pb uptake was increased in the presence of BDE-209, but BDE-209 bioconcentration and the ability to metabolize BDE-209 were decreased in the presence of Pb. These results suggest that BDE-209 and Pb have a synergistic disruptive effect on neurodevelopment in zebrafish larvae by enhanced generation of ROS, which is a major factor that contributes to developmental neurotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping