|ZFIN ID: ZDB-PUB-151019-9|
Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development
Sun, G., Liu, K., Wang, X., Liu, X., He, Q., Hsiao, C.D.
|Source:||Molecules 20: 18539-18550 (Journal)|
|Registered Authors:||Hsiao, Chung-Der|
|Keywords:||E-selectin, expression, identification, lipopolysaccharide, zebrafish|
|PubMed:||26473817 Full text @ Molecules|
Sun, G., Liu, K., Wang, X., Liu, X., He, Q., Hsiao, C.D. (2015) Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development. Molecules. 20:18539-18550.
ABSTRACTIn this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.