ZFIN ID: ZDB-PUB-151019-9
Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development
Sun, G., Liu, K., Wang, X., Liu, X., He, Q., Hsiao, C.D.
Date: 2015
Source: Molecules   20: 18539-18550 (Journal)
Registered Authors: Hsiao, Chung-Der
Keywords: E-selectin, expression, identification, lipopolysaccharide, zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Conserved Sequence
  • E-Selectin/genetics
  • E-Selectin/immunology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/immunology
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Endothelial Cells/cytology
  • Endothelial Cells/drug effects
  • Endothelial Cells/immunology
  • Gene Expression Regulation, Developmental*
  • Hematopoiesis/drug effects
  • Hematopoiesis/genetics*
  • Hematopoiesis/immunology
  • Humans
  • Inflammation
  • Interleukin-1beta/genetics
  • Interleukin-1beta/immunology
  • Lipopolysaccharides/pharmacology
  • Mice
  • Molecular Sequence Data
  • Neovascularization, Physiologic/drug effects
  • Neovascularization, Physiologic/genetics*
  • Neovascularization, Physiologic/immunology
  • Open Reading Frames
  • Phylogeny
  • Sequence Alignment
  • Stem Cells/cytology
  • Stem Cells/drug effects
  • Stem Cells/immunology
  • Tumor Necrosis Factor-alpha/genetics
  • Tumor Necrosis Factor-alpha/immunology
  • Zebrafish/classification
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/immunology
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology
PubMed: 26473817 Full text @ Molecules
In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.