PUBLICATION
Structural and functional characterization of neuromedin S in the teleost fish, zebrafish (Danio rerio)
- Authors
- Chen, H., Huang, H., Chen, X., Deng, S., Zhu, C., Huang, H., Li, G.
- ID
- ZDB-PUB-150930-8
- Date
- 2016
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 191: 76-83 (Journal)
- Registered Authors
- Keywords
- feeding, neuroendocrine regulation, neuromedin S, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/cytology
- Brain/metabolism
- Cloning, Molecular
- Food Deprivation
- Gene Expression Regulation
- Humans
- Mice
- Molecular Sequence Data
- Neuropeptides/chemistry*
- Neuropeptides/genetics
- Neuropeptides/metabolism*
- Orexins/genetics
- Protein Transport
- Sequence Analysis
- Zebrafish*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 26415865 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Chen, H., Huang, H., Chen, X., Deng, S., Zhu, C., Huang, H., Li, G. (2016) Structural and functional characterization of neuromedin S in the teleost fish, zebrafish (Danio rerio). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 191:76-83.
Abstract
Neuromedin S (NMS) has been demonstrated to have important roles in many vertebrate physiological processes. However, the function of NMS in teleost fishes remains unclear. We explored the physiological roles of the NMS gene in the zebrafish model. An NMS cDNA was cloned from zebrafish brain tissue, and the full length cDNA sequence was 521 bp in length and encoded a precursor of 110 amino acid residues. Interestingly, fish prepro-NMS is predicted to generate a short 34-residue peptide, designated as NMS-related peptide (NMSRP). Zebrafish prepro-NMS does not contain the NMS peptide which found in the NMS precursors of mammals, and just retains the MNSRP peptide. A multiple species sequence alignment showed that NMSRPs are conserved among the other sampled vertebrates. Zebrafish NMS mRNA was detected by RT-PCR revealing a tissue-specific distribution with high levels of expression in the brain, spleen, ovary, pituitary, and muscle. Furthermore, the locations of NMS-expressing cells in the zebrafish brain were detected by in situ hybridization in the parvocellularpreoptic nucleus (PPa), the ventral zone of the periventricular hypothalamus (Hv), and lateral hypothalamic nucleus (LH). The levels of NMS mRNA in the hypothalamus were significantly increased after three days of food-deprivation. Administration of zebrafish NMSRP by intraperitoneal injection significantly promoted the expression of neuropeptide Y (NPY) and orexin, suggesting anorexigenic role for NMSRP in zebrafish. The present study offers a new understanding of the NMS gene in vertebrates and increases our knowledge of the neuroendocrine regulation of feeding.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping