|ZFIN ID: ZDB-PUB-150926-1|
Fast 3-D temporal focusing microscopy using an electrically tunable lens
Jiang, J., Zhang, D., Walker, S., Gu, C., Ke, Y., Yung, W.H., Chen, S.C.
|Source:||Optics express 23: 24362-24368 (Journal)|
|Registered Authors:||Walker, Steven|
|PubMed:||26406641 Full text @ Opt. Express|
Jiang, J., Zhang, D., Walker, S., Gu, C., Ke, Y., Yung, W.H., Chen, S.C. (2015) Fast 3-D temporal focusing microscopy using an electrically tunable lens. Optics express. 23:24362-24368.
ABSTRACTIn this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca2+ imaging of neurons in GaCamp6 labeled zebrafish was performed.