|ZFIN ID: ZDB-PUB-150919-1|
Diabetic pdx1-mutant zebrafish show conserved responses to nutrient overload and anti-glycemic treatment
Kimmel, R.A., Dobler, S., Schmitner, N., Walsen, T., Freudenblum, J., Meyer, D.
|Source:||Scientific Reports 5: 14241 (Journal)|
|Registered Authors:||Kimmel, Robin, Meyer, Dirk, Schmitner, Nicole, Walsen, Tanja|
|PubMed:||26384018 Full text @ Sci. Rep.|
Kimmel, R.A., Dobler, S., Schmitner, N., Walsen, T., Freudenblum, J., Meyer, D. (2015) Diabetic pdx1-mutant zebrafish show conserved responses to nutrient overload and anti-glycemic treatment. Scientific Reports. 5:14241.
ABSTRACTDiabetes mellitus is characterized by disrupted glucose homeostasis due to loss or dysfunction of insulin-producing beta cells. In this work, we characterize pancreatic islet development and function in zebrafish mutant for pdx1, a gene which in humans is linked to genetic forms of diabetes and is associated with increased susceptibility to Type 2 diabetes. Pdx1 mutant zebrafish have the key diabetic features of reduced beta cells, decreased insulin and elevated glucose. The hyperglycemia responds to pharmacologic anti-diabetic treatment and, as often seen in mammalian diabetes models, beta cells of pdx1 mutants show sensitivity to nutrient overload. This unique genetic model of diabetes provides a new tool for elucidating the mechanisms behind hyperglycemic pathologies and will allow the testing of novel therapeutic interventions in a model organism that is amenable to high-throughput approaches.