PUBLICATION
Application of chrysophanol in zebrafish to reduce dietary introduced lipid and its possible mechanism
- Authors
- Chen, K., Wang, C.Q., Fan, Y.Q., Xie, Y.S., Yin, Z.F., Xu, Z.J., Zhang, H.L., Cao, J.T., Wang, Y.
- ID
- ZDB-PUB-150918-1
- Date
- 2015
- Source
- International journal of clinical and experimental medicine 8(7): 10558-10567 (Journal)
- Registered Authors
- Keywords
- Chrysophanol, atorvastain, lipid-lowering effect, zebrafish
- MeSH Terms
- none
- PubMed
- 26379845
Citation
Chen, K., Wang, C.Q., Fan, Y.Q., Xie, Y.S., Yin, Z.F., Xu, Z.J., Zhang, H.L., Cao, J.T., Wang, Y. (2015) Application of chrysophanol in zebrafish to reduce dietary introduced lipid and its possible mechanism. International journal of clinical and experimental medicine. 8(7):10558-10567.
Abstract
Purpose To explore the therapeutic potential and mechanism of chrysophanol on lipid-lowering function.
Methods Zebrafish or larvae were employed to evaluate the effect of chrysophanol on lipid-lowering. Zebrafish of 5 day post fertilization (dpf, larva) and 13-week old were fed with high-cholesterol diet or high-fat to investigate the influence of chrysophanol comparing with atorvastain and co-administration of chrysophanol and atorvastain on subsistent blood lipid using the fluorescence microscope and lipid panel screen. Thereafter, we enrolled zebrafish of 7 dpf fed with high-fat diet to explore the lipid-lowering mechanism of chrysophanol basing on the frequency of peristalsis and the residual on the digestive wall.
Results Chrysophanol could significantly lower cholesterol both in zebrafish and larvae (P < 0.05), and the co-administration of chrysophanol and atorvastatin had the best performance in reducing cholesterol (P < 0.05). Chrysophanol and atorvastain could also significantly lower triglyceride. Moreover, we found that chrysophanol attached on the digestive wall for a long time and enhanced the frequency of peristalsis.
Conclusions Chrysophanol has lipid-lowering effect both in zebrafish and larvae which may be attributed to the effect on the frequency of peristalsis and fat absorption, and co-administration with atorvastain performs better lipid-lowering effect in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping