PUBLICATION
DNA Vaccination Boosts Bacillus Calmette-Guérin Protection against Mycobacterial Infection in Zebrafish
- Authors
- Oksanen, K.E., Myllymäki, H., Ahava, M.J., Mäkinen, L., Parikka, M., Rämet, M.
- ID
- ZDB-PUB-150913-2
- Date
- 2016
- Source
- Developmental and comparative immunology 54(1): 89-96 (Journal)
- Registered Authors
- Keywords
- BCG, Mycobacterium marinum, tuberculosis, vaccine, zebrafish
- MeSH Terms
-
- Animals
- BCG Vaccine/immunology*
- Disease Models, Animal
- Mycobacterium Infections, Nontuberculous/prevention & control*
- Mycobacterium marinum
- Polymerase Chain Reaction
- Vaccines, DNA/immunology*
- Zebrafish
- PubMed
- 26363085 Full text @ Dev. Comp. Immunol.
Citation
Oksanen, K.E., Myllymäki, H., Ahava, M.J., Mäkinen, L., Parikka, M., Rämet, M. (2016) DNA Vaccination Boosts Bacillus Calmette-Guérin Protection against Mycobacterial Infection in Zebrafish. Developmental and comparative immunology. 54(1):89-96.
Abstract
Despite the widespread use of the current Bacillus Calmette-Guérin (BCG) vaccine, tuberculosis is still a major cause of morbidity and mortality worldwide. Vaccination with BCG does not prevent a Mycobacterium tuberculosis infection, nor does it inhibit the reactivation of latent tuberculosis. Here, we show that adult zebrafish are modestly and variably protected from a mycobacterial infection by BCG vaccination. An intraperitoneal (i.p.) BCG vaccination was associated with enhanced survival upon a high-dose (20,000 bacteria) Mycobacterium marinum infection. In addition, BCG-vaccinated fish were more able to restrict a low-dose (30 bacteria) intraperitoneal infection with M. marinum, as indicated by lower bacterial loads at six weeks post infection (wpi). However, the vaccination could not completely prevent an infection. A qRT-PCR analysis comparing BCG-vaccinated and unvaccinated fish upon a mycobacterial infection indicated that the induction of Tumor necrosis factor (TNF) was more modest in vaccinated fish. The partial protection gained by BCG could be boosted by a DNA vaccine combining Ag85B, ESAT6 and a resuscitation-related gene RpfE, suggesting that this combination of antigens could be useful for a future BCG booster vaccine. We conclude that zebrafish is a useful early-phase preclinical model for studying subunit vaccines designed for boosting the effects of BCG.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping