PUBLICATION
Bioconcentration, metabolism and alterations of thyroid hormones of Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) in Zebrafish
- Authors
- Xu, T., Wang, Q., Shi, Q., Fang, Q., Guo, Y., Zhou, B.
- ID
- ZDB-PUB-150912-9
- Date
- 2015
- Source
- Environmental Toxicology and Pharmacology 40: 581-586 (Journal)
- Registered Authors
- Keywords
- Bioconcentration and metabolism, TDCPP, Thyroid hormones, Zebrafish
- MeSH Terms
-
- Animals
- Cytochrome P-450 Enzyme System/genetics
- Female
- Gene Expression Regulation, Enzymologic/drug effects*
- Liver/drug effects
- Liver/metabolism
- Organophosphorus Compounds/analysis*
- Organophosphorus Compounds/toxicity
- Thyroid Hormones/blood*
- Thyroxine/blood
- Triiodothyronine/blood
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- PubMed
- 26356387 Full text @ Environ. Toxicol. Pharmacol.
- CTD
- 26356387
Citation
Xu, T., Wang, Q., Shi, Q., Fang, Q., Guo, Y., Zhou, B. (2015) Bioconcentration, metabolism and alterations of thyroid hormones of Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) in Zebrafish. Environmental Toxicology and Pharmacology. 40:581-586.
Abstract
Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is an organophosphate widely used as a flame retardant, and has frequently been detected in the environment and biota. The present study investigates the bioconcentration and metabolism of TDCPP in fish. Zebrafish embryos (from 2h post-fertilization) were exposed to TDCPP (0, 4, 20 and 100μg/L) for six months. Exposure to TDCPP significantly induced phase I metabolic enzymes 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) in fish. The mRNA expression of genes related to Phase I and II metabolic enzymes, such as cyp1a1, cyp1b1, cyp1c1 and ugt1ab were also significantly upregulated. Exposure to TDCPP significantly reduced plasma thyroxine (T4) and 3,5,3'-triiodothyronine (T3) levels in females. Chemicals analysis indicated significant levels of TDCPP and its metabolite, bis (1,3-dichloro-2-propyl) phosphate (BDCPP), in the liver. The present study reveals that exposure to low concentrations of TDCPP can cause bioconcentration in fish, and TDCPP can be readily metabolized in liver.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping