|ZFIN ID: ZDB-PUB-150912-13|
Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development
Uribe, R.A., Bronner, M.E.
|Source:||Molecular biology of the cell 26(21): 3728-40 (Journal)|
|Registered Authors:||Bronner-Fraser, Marianne, Uribe, Rosa|
|PubMed:||26354419 Full text @ Mol. Biol. Cell|
Uribe, R.A., Bronner, M.E. (2015) Meis3 is required for neural crest invasion of the gut during zebrafish enteric nervous system development. Molecular biology of the cell. 26(21):3728-40.
ABSTRACTDuring development, vagal neural crest cells fated to contribute to the enteric nervous system migrate ventrally away from the neural tube toward and along the primitive gut. The molecular mechanisms that regulate their early migration en route to and entry into the gut remain elusive. Here, we show that the transcription factor meis3 is expressed along vagal neural crest pathways. Meis3 loss of function results in a reduction in migration efficiency, cell number and the mitotic activity of neural crest cells in the vicinity of the gut, while having no effect on neural crest or gut specification. Later, during enteric nervous system differentiation, Meis3 depleted embryos exhibit colonic aganglionosis, a disorder in which the hindgut is devoid of neurons. Accordingly, the expression of Shh pathway components, previously shown to have a role in the etiology of Hirschsprung's disease, was misregulated within the gut following loss of Meis3. Taken together, these findings support a model in which Meis3 is required for neural crest proliferation, migration into and colonization of the gut such that its loss leads to severe defects in enteric nervous system development.