ZFIN ID: ZDB-PUB-150904-2
Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4
Hayward, A.G., Joshi, P., Skromne, I.
Date: 2015
Source: Developmental dynamics : an official publication of the American Association of Anatomists   244(12): 1564-73 (Journal)
Registered Authors: Skromne, Isaac
Keywords: Temporal collinearity, axial patterning, spatial collinearity
MeSH Terms:
  • Animals
  • Body Patterning/genetics*
  • Gene Expression Regulation, Developmental*
  • Genes, Homeobox/genetics*
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism
  • Transcriptional Activation
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 26335559 Full text @ Dev. Dyn.
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ABSTRACT
Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this question by analyzing the spatiotemporal expression profile of all forty-nine zebrafish hox genes in wild type and Cdx4-deficient embryos.
Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tailbud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase.
These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase. This article is protected by copyright. All rights reserved.
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