ZFIN ID: ZDB-PUB-150901-12
Effect of acetochlor on transcription of genes associated with oxidative stress, apoptosis, immunotoxicity and endocrine disruption in the early life stage of zebrafish
Jiang, J., Wu, S., Liu, X., Wang, Y., An, X., Cai, L., Zhao, X.
Date: 2015
Source: Environmental Toxicology and Pharmacology   40: 516-523 (Journal)
Registered Authors:
Keywords: Acetochlor, Cell apoptosis, Endocrine disruption, Immunotoxicity, Oxidative stress, Zebrafish
MeSH Terms:
  • Animals
  • Apoptosis
  • Embryo, Nonmammalian/drug effects
  • Endocrine Disruptors/toxicity
  • Gene Expression Regulation, Developmental/drug effects
  • Gonads/drug effects
  • Herbicides/toxicity*
  • Immunity, Innate/drug effects*
  • Oxidative Stress/drug effects
  • Toluidines/toxicity*
  • Transcription, Genetic/drug effects*
  • Up-Regulation
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 26318563 Full text @ Environ. Toxicol. Pharmacol.
ABSTRACT
The study presented here aimed to characterize the effects of acetochlor on expression of genes related to endocrine disruption, oxidative stress, apoptosis and immune system in zebrafish during its embryo development. Different trends in gene expression were observed after exposure to 50, 100, 200μg/L acetochlor for 96h. Results demonstrated that the transcription patterns of many key genes involved in the hypothalamic-pituitary-gonadal/thyroid (HPG/HPT) axis (e.g., VTG1, ERβ1, CYP19a and TRα), cell apoptosis pathway (e.g., Bcl2, Bax, P53 and Cas8), as well as innate immunity (e.g., CXCL-C1C, IL-1β and TNFα) were affected in newly hatched zebrafish after exposure to acetochlor. In addition, the up-regulation of CAT, GPX, GPX1a, Cu/Zn-SOD and Ogg1 suggested acetochlor might trigger oxidative stress in zebrafish. These finding indicated that acetochlor could simultaneously induce multiple responses during zebrafish embryonic development, and bidirectional interactions among oxidative stress, apoptosis pathway, immune and endocrine systems might be present.
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